Charge and topography patterned lithium niobate provides physical cues to fluidically isolated cortical axons
2017 (English)In: Applied Physics Letters, ISSN 0003-6951, E-ISSN 1077-3118, Vol. 110, no 053702Article in journal (Refereed) Published
In vitro devices that combine chemotactic and physical cues are needed for understanding how cells integrate different stimuli. We explored the suitability of lithium niobate (LiNbO3), a transparent ferroelectric material that can be patterned with electrical charge domains and micro/ nanotopography, as a neural substrate. On flat LiNbO3 z-surfaces with periodically alternating charge domains, cortical axons are partially aligned with domain boundaries. On submicron-deep etched trenches, neurites are aligned with the edges of the topographical features. Finally, we bonded a bicompartmental microfluidic chip to LiNbO3 surfaces patterned by etching, to create isolated axon microenvironments with predefined topographical cues. LiNbO3 is shown to be an emerging neuron culture substrate with tunable electrical and topographical properties that can be integrated with microfluidic devices, suitable for studying axon growth and guidance mechanisms under combined topographical/chemical stimuli.
Place, publisher, year, edition, pages
2017. Vol. 110, no 053702
Axons, Topography, Fluidic devices, Etching, Atomic force microscopy
Other Physics Topics
IdentifiersURN: urn:nbn:se:kth:diva-203961DOI: 10.1063/1.4975304OAI: oai:DiVA.org:kth-203961DiVA: diva2:1083290