Size-selective colloidal-gold localization in transmission x-ray microscopy
2007 (English)In: Journal of Microscopy, ISSN 0022-2720, E-ISSN 1365-2818, Vol. 225, no 1, 80-87 p.Article in journal (Refereed) Published
Colloidal gold is a useful marker for functional-imaging experiments in transmission X-ray microscopy. Due to the low contrast of gold particles with small diameters it is necessary to develop a powerful algorithm to localize the single gold particles. The presented image-analysis algorithm for identifying colloidal gold particles is based on the combination of a threshold with respect to the local absorption and shape discrimination, realized by fitting a Gaussian profile to the identified regions of interest. The shape discrimination provides the possibility of size-selective identification and localization of single colloidal gold particles down to a diameter of 50 nm. The image-analysis algorithm, therefore, has potential for localization studies of several proteins simultaneously and for localization of fiducial markers in X-ray tomography.
Place, publisher, year, edition, pages
2007. Vol. 225, no 1, 80-87 p.
Colloidal gold; Image analysis; Soft X-ray microscopy; algorithm; article; image analysis; microscopy; normal distribution; particle size; priority journal; protein localization; transmission X ray microscopy; X ray microanalysis
IdentifiersURN: urn:nbn:se:kth:diva-6201DOI: 10.1111/j.1365-2818.2007.01717.xISI: 000243974000008ScopusID: 2-s2.0-33846829091OAI: oai:DiVA.org:kth-6201DiVA: diva2:10845
QC 20100820. Uppdaterad från Accepted till Published 20100820.2006-10-032006-10-032010-08-20Bibliographically approved