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Epitopes of anti-RIFIN antibodies and characterization of rif-expressing Plasmodium falciparum parasites by RNA sequencing
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0003-1242-0873
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2017 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 7, 43190Article in journal (Refereed) Published
Abstract [en]

Variable surface antigens of Plasmodium falciparum have been a major research focus since they facilitate parasite sequestration and give rise to deadly malaria complications. Coupled with its potential use as a vaccine candidate, the recent suggestion that the repetitive interspersed families of polypeptides (RIFINs) mediate blood group A rosetting and influence blood group distribution has raised the research profile of these adhesins. Nevertheless, detailed investigations into the functions of this highly diverse multigene family remain hampered by the limited number of validated reagents. In this study, we assess the specificities of three promising polyclonal anti-RIFIN antibodies that were IgG-purified from sera of immunized animals. Their epitope regions were mapped using a 175,000-peptide microarray holding overlapping peptides of the P. falciparum variable surface antigens. Through immunoblotting and immunofluorescence imaging, we show that different antibodies give varying results in different applications/assays. Finally, we authenticate the antibody-based detection of RIFINs in two previously uncharacterized non-rosetting parasite lines by identifying the dominant rif transcripts using RNA sequencing.

Place, publisher, year, edition, pages
Nature Publishing Group, 2017. Vol. 7, 43190
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Basic Medicine
Identifiers
URN: urn:nbn:se:kth:diva-204077DOI: 10.1038/srep43190ISI: 000394930400001PubMedID: 28233866ScopusID: 2-s2.0-85013755282OAI: oai:DiVA.org:kth-204077DiVA: diva2:1085479
Note

QC 20170329

Available from: 2017-03-29 Created: 2017-03-29 Last updated: 2017-03-29Bibliographically approved

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Zandian, ArashNilsson, PeterQundos, Ulrika
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CiteExportLink to record
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