Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
MicroBubble Activated Acoustic Cell Sorting: BAACS
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab. mafaridi@kth.se. (Clinical Microfluidics)ORCID iD: 0000-0003-1176-0905
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab. (Clinical Microfluidics)ORCID iD: 0000-0001-5199-0663
KTH, School of Engineering Sciences (SCI), Applied Physics.ORCID iD: 0000-0003-0064-0086
KTH, School of Technology and Health (STH), Medical Engineering, Medical Imaging.ORCID iD: 0000-0002-3699-396X
Show others and affiliations
2017 (English)In: Biomedical microdevices (Print), ISSN 1387-2176, E-ISSN 1572-8781, Vol. 19, no 2Article in journal (Refereed) Published
Abstract [en]

Acoustophoresis, the ability to acoustically manipulate particles and cells inside a microfluidic channel, is a critical enabling technology for cell-sorting applications. However, one of the major impediments for routine use of acoustophoresis at clinical laboratory has been the reliance on the inherent physical properties of cells for separation. Here, we present a microfluidic-based microBubble-Activated Acoustic Cell Sorting (BAACS) method that rely on the specific binding of target cells to microbubbles conjugated with specific antibodies on their surface for continuous cell separation using ultrasonic standing wave. In acoustophoresis, cells being positive acoustic contrast particles migrate to pressure nodes. On the contrary we show that air-filled polymer-shelled microbubbles being strong negative acoustic contrast particles migrate to pressure antinodes at acoustic pressure amplitudes as low as 60 kPa. As a proof of principle, using the BAACS strategy, we demonstrate the separation of cancer cell line in a suspension with better than 75% efficiency. Moreover, 100% of the microbubble-cell conjugates migrated to the anti-node. Hence a better upstream affinity-capture has the potential to provide higher sorting efficiency. The BAACS technique may potentially provide a simplistic approach for similar sized selective isolation of cells, and is suited for applications in point of care.

Place, publisher, year, edition, pages
Springer, 2017. Vol. 19, no 2
Keyword [en]
Cell sorting, acoustophoresis, microbubble, contrast agent, microfluidic separation
National Category
Medical Biotechnology
Identifiers
URN: urn:nbn:se:kth:diva-205293DOI: 10.1007/s10544-017-0157-4Scopus ID: 2-s2.0-85016958658OAI: oai:DiVA.org:kth-205293DiVA: diva2:1088338
Funder
EU, FP7, Seventh Framework Programme, 115153
Note

QC 20170515

Available from: 2017-04-12 Created: 2017-04-12 Last updated: 2017-05-19Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Faridi, Muhammad AsimRamachandraiah, HarishaIranmanesh, Ida SadatGrishenkov, DmitryWiklund, MartinRussom, Aman
By organisation
Proteomics and NanobiotechnologyScience for Life Laboratory, SciLifeLabApplied PhysicsMedical ImagingBiomedical and X-ray Physics
In the same journal
Biomedical microdevices (Print)
Medical Biotechnology

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 28 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf