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Antibody validation by immunoprecipitation followed by mass spectrometry analysis
KTH, School of Biotechnology (BIO), Protein Technology. KTH, Centres, Science for Life Laboratory, SciLifeLab. (Drug Discovery and Development Platform)
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2017 (English)In: Methods in Molecular Biology, Humana Press Inc. , 2017, p. 175-187Conference paper, Published paper (Refereed)
Abstract [en]

We describe a mass spectrometry-based approach for validation of antibody specificity. This method allows validation of antibodies or antibody fragments, against their endogenous targets. It can assess if the antibody is able to bind to its native antigen in cell lysates among thousands of other proteins, DNA, RNA, and other cellular components. In addition, it identifies other proteins the antibody is able to immunoprecipitate allowing for the assessment of antibody specificity and selectivity. This method is easily scalable, adaptable to different cell lines and conditions and has been shown to be reproducible between multiple laboratories.

Place, publisher, year, edition, pages
Humana Press Inc. , 2017. p. 175-187
Keywords [en]
Antibody fragments, Antibody validation, Immunoprecipitation, Mass spectrometry
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:kth:diva-207430DOI: 10.1007/978-1-4939-6857-2_10Scopus ID: 2-s2.0-85014733708OAI: oai:DiVA.org:kth-207430DiVA, id: diva2:1098275
Note

QC 20170523

Available from: 2017-05-23 Created: 2017-05-23 Last updated: 2017-05-23Bibliographically approved

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Protein TechnologyScience for Life Laboratory, SciLifeLab
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