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Colonization and Succession within the Human Gut Microbiome by Archaea, Bacteria, and Microeukaryotes during the First Year of Life
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2017 (English)In: Frontiers in Microbiology, ISSN 1664-302X, E-ISSN 1664-302X, Vol. 8, article id 738Article in journal (Refereed) Published
Abstract [en]

Perturbations to the colonization process of the human gastrointestinal tract have been suggested to result in adverse health effects later in life. Although much research has been performed on bacterial colonization and succession, much less is known about the other two domains of life, archaea, and eukaryotes. Here we describe colonization and succession by bacteria, archaea and microeukaryotes during the first year of life (samples collected around days 1, 3, 5, 28, 150, and 365) within the gastrointestinal tract of infants delivered either vaginally or by cesarean section and using a combination of quantitative real-time PCR as well as 16S and 18S rRNA gene amplicon sequencing. Sequences from organisms belonging to all three domains of life were detectable in all of the collected meconium samples. The microeukaryotic community composition fluctuated strongly over time and early diversification was delayed in infants receiving formula milk. Cesarean section-delivered (CSD) infants experienced a delay in colonization and succession, which was observed for all three domains of life. Shifts in prokaryotic succession in CSD infants compared to vaginally delivered (VD) infants were apparent as early as days 3 and 5, which were characterized by increased relative abundances of the genera Streptococcus and Staphylococcus, and a decrease in relative abundance for the genera Bifidobacterium and Bacteroides. Generally, a depletion in Bacteroidetes was detected as early as day 5 postpartum in CSD infants, causing a significantly increased Firmicutes/Bacteroidetes ratio between days 5 and 150 when compared to VD infants. Although the delivery mode appeared to have the strongest influence on differences between the infants, other factors such as a younger gestational age or maternal antibiotics intake likely contributed to the observed patterns as well. Our findings complement previous observations of a delay in colonization and succession of CSD infants, which affects not only bacteria but also archaea and microeukaryotes. This further highlights the need for resolving bacterial, archaeal, and microeukaryotic dynamics in future longitudinal studies of microbial colonization and succession within the neonatal gastrointestinal tract.

Place, publisher, year, edition, pages
Frontiers Research Foundation , 2017. Vol. 8, article id 738
Keywords [en]
fungi, succession, delivery mode, infant gut microbiome, amplicon sequencing, microbial colonization, quantitative real-time PCR
National Category
Pediatrics Microbiology
Identifiers
URN: urn:nbn:se:kth:diva-207887DOI: 10.3389/fmicb.2017.00738ISI: 000400339800001PubMedID: 28512451Scopus ID: 2-s2.0-85019737857OAI: oai:DiVA.org:kth-207887DiVA, id: diva2:1103542
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20170530

Available from: 2017-05-30 Created: 2017-05-30 Last updated: 2017-11-29Bibliographically approved

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Hugerth, LuisaAndersson, Anders F.

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