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High throughput droplet sorting of yeast for p-Coumaric acid production detected by co-encapsulated E. coli biosensor bacteria
KTH.
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2016 (English)In: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Chemical and Biological Microsystems Society , 2016, 551-552 p.Conference paper, Published paper (Refereed)
Abstract [en]

We present the optimization of E. coli biosensor bacteria and demonstrate their use to detect and sort co-encapsulated S. cerevisiae yeast cells by their p-Coumaric acid (pCA) production in picoliter microfluidic droplets at high throughput. This strategy allows us to enrich pCA producing cell factories by the cell permeable product pCA using a biosensor in a separate cell.

Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society , 2016. 551-552 p.
Keyword [en]
Biosensors, Droplet microfluidics, High throughput screening, Synthetic biology
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-207552Scopus ID: 2-s2.0-85014274649ISBN: 9780979806490 (print)OAI: oai:DiVA.org:kth-207552DiVA: diva2:1103769
Conference
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, 9 October 2016 through 13 October 2016
Note

Conference code: 126047; Export Date: 22 May 2017; Conference Paper; Correspondence Address: Joensson, H.N.; KTH Royal Institute of TechnologySweden; email: hakan.jonsson@biotech.kth.se. QC 20170531

Available from: 2017-05-31 Created: 2017-05-31 Last updated: 2017-05-31Bibliographically approved

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Jönsson, Håkan

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