Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Scanning electron microscopy (SEM) protocols for problematic plant, oomycete, and fungal samples
KTH, School of Biotechnology (BIO), Glycoscience.
Show others and affiliations
2017 (English)In: Journal of Visualized Experiments, ISSN 1940-087X, E-ISSN 1940-087X, Vol. 2017, no 120, e55031Article in journal (Refereed) Published
Abstract [en]

Common problems in the processing of biological samples for observations with the scanning electron microscope (SEM) include cell collapse, treatment of samples from wet microenvironments and cell destruction. Using young floral tissues, oomycete cysts, and fungi spores (Agaricalesas examples, specific protocols to process delicate samples are described here that overcome some of the main challenges in sample treatment for image capture under the SEM. Floral meristems fixed with FAA (Formalin-Acetic-Alcohol) and processed with the Critical Point Dryer (CPD) did not display collapsed cellular walls or distorted organs. These results are crucial for the reconstruction of floral development. A similar CPD-based treatment of samples from wet microenvironments, such as the glutaraldehyde-fixed oomycete cysts, is optimal to test the differential growth of diagnostic characteristics (e.g., the cyst spines) on different types of substrates. Destruction of nurse cells attached to fungi spores was avoided after rehydration, dehydration, and the CPD treatment, an important step for further functional studies of these cells. The protocols detailed here represent low-cost and rapid alternatives for the acquisition of good-quality images to reconstruct growth processes and to study diagnostic characteristics.

Place, publisher, year, edition, pages
Journal of Visualized Experiments , 2017. Vol. 2017, no 120, e55031
Keyword [en]
Agaricales, Critical point dryer, Cysts, Formaldehyde, Glutaraldehyde, Issue 120, Phellorinia, Plant biology, Plant development, Saprolegnia, Sputter coater
National Category
Other Medical Biotechnology
Identifiers
URN: urn:nbn:se:kth:diva-208129DOI: 10.3791/55031ISI: 000397847700036Scopus ID: 2-s2.0-85014381669OAI: oai:DiVA.org:kth-208129DiVA: diva2:1106127
Funder
EU, Horizon 2020, 634429
Note

QC 20170607

Available from: 2017-06-07 Created: 2017-06-07 Last updated: 2017-06-07Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Rezinciuc, Svetlana
By organisation
Glycoscience
In the same journal
Journal of Visualized Experiments
Other Medical Biotechnology

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 19 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf