Study of Real-time Lectin–Carbohydrate Interactions on the Surface of a Quartz Crystal Microbalance
2005 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 21, no 1, 60-66 p.Article in journal (Refereed) Published
A quartz crystal microbalance (QCM) biosensor system for lectin-carbohydrate interactions has been developed. Yeast mannan was immobilised on polystyrene-coated quartz crystals, and interactions tested with the lectin concanavalin A (Con A). The biosensor could be easily operated, where mannan immobilisation and all binding analyses were performed in real-time using a flow-through system. The apparent binding constant for yeast mannan to Con A was estimated to be 0.4 μM, well in accordance to reported literature values. In addition, the effective concentration values (EC50-values) for a series of mannose/mannoside ligands, acting as competitors to the mannan/Con A interaction, were determined to range from 0.18 to 5.3 mM, in good correlation with a related enzyme-labelled lectin assay (ELLA) protocol
Place, publisher, year, edition, pages
2005. Vol. 21, no 1, 60-66 p.
Carbohydrates, Glycochemistry, Lectins; Molecular recognition, Quartz crystal microbalance
IdentifiersURN: urn:nbn:se:kth:diva-6389DOI: 10.1016/j.bios.2004.10.006ISI: 000232268900006ScopusID: 2-s2.0-20444439074OAI: oai:DiVA.org:kth-6389DiVA: diva2:11085
QC 201009152006-11-222006-11-222010-09-15Bibliographically approved