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Study of Real-time Lectin–Carbohydrate Interactions on the Surface of a Quartz Crystal Microbalance
KTH, School of Chemical Science and Engineering (CHE), Chemistry.
KTH, School of Chemical Science and Engineering (CHE), Chemistry.
2005 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 21, no 1, 60-66 p.Article in journal (Refereed) Published
Abstract [en]

A quartz crystal microbalance (QCM) biosensor system for lectin-carbohydrate interactions has been developed. Yeast mannan was immobilised on polystyrene-coated quartz crystals, and interactions tested with the lectin concanavalin A (Con A). The biosensor could be easily operated, where mannan immobilisation and all binding analyses were performed in real-time using a flow-through system. The apparent binding constant for yeast mannan to Con A was estimated to be 0.4 μM, well in accordance to reported literature values. In addition, the effective concentration values (EC50-values) for a series of mannose/mannoside ligands, acting as competitors to the mannan/Con A interaction, were determined to range from 0.18 to 5.3 mM, in good correlation with a related enzyme-labelled lectin assay (ELLA) protocol

Place, publisher, year, edition, pages
2005. Vol. 21, no 1, 60-66 p.
Keyword [en]
Carbohydrates, Glycochemistry, Lectins; Molecular recognition, Quartz crystal microbalance
National Category
Organic Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-6389DOI: 10.1016/j.bios.2004.10.006ISI: 000232268900006Scopus ID: 2-s2.0-20444439074OAI: oai:DiVA.org:kth-6389DiVA: diva2:11085
Note
QC 20100915Available from: 2006-11-22 Created: 2006-11-22 Last updated: 2010-09-15Bibliographically approved
In thesis
1. Carbohydrate Synthesis and Study of Carbohydrate-Lectin Interactions Using QCM Biosensors and Microarray Technologies
Open this publication in new window or tab >>Carbohydrate Synthesis and Study of Carbohydrate-Lectin Interactions Using QCM Biosensors and Microarray Technologies
2006 (English)Doctoral thesis, comprehensive summary (Other scientific)
Abstract [en]

Interactions between carbohydrates and proteins are increasingly being recognized as crucial in many biological processes, such as cellular adhesion and communication. In order to investigate the interactions of carbohydrates and proteins, the development of efficient analytic technologies, as well as novel strategies for the synthesis of carbohydrates, have to be explored. To date, several methods have been exploited to analyze interactions of carbohydrates and proteins, for example, biosensors, nuclear magnetic resonance (NMR); enzyme-linked immunosorbent assays (ELISA), X-ray crystallography and array technologies. This thesis describes the development of novel strategies for the synthesis of carbohydrates, as well as new efficient strategies to Quartz Crystal Microbalance- (QCM-) biosensors and carbohydrate microarrays technologies. These methodologies have been used to probe carbohydrate-lectin-interactions for a range of plant and animal lectins.

Place, publisher, year, edition, pages
Stockholm: KTH, 2006. 106 p.
Series
Trita-IOK, ISSN 1100-7974 ; 2006:106
Keyword
Lectins; Carbohydrates; Molecular recognition; Biosensors; Quartz crystal microbalance; Carbohydrate microarrays.
National Category
Organic Chemistry
Identifiers
urn:nbn:se:kth:diva-4177 (URN)91-7178-503-5 (ISBN)
Public defence
2006-12-01, D3, Lindstedtsvägen 5, Stockholm, 10:00
Opponent
Supervisors
Note
QC 20100915Available from: 2006-11-22 Created: 2006-11-22 Last updated: 2011-09-05Bibliographically approved

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Publisher's full textScopushttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TFC-4DVBJ9B-2&_user=4478132&_coverDate=07%2F15%2F2005&_alid=493485618&_rdoc=1&_fmt=summary&_orig=search&_cdi=5223&_sort=d&_docanchor=&view=c&_acct=C000034958&_version=1&_urlVersion=0&_userid=4478132&md5=3949377cbdf6f7b3676561e935fc818d

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