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Photogenerated Carbohydrate Microarrays
KTH, School of Chemical Science and Engineering (CHE), Chemistry.
KTH, School of Chemical Science and Engineering (CHE), Chemistry.
KTH, School of Chemical Science and Engineering (CHE), Chemistry.
KTH, School of Biotechnology (BIO).
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2007 (English)In: ChemBioChem (Print), ISSN 1439-4227, E-ISSN 1439-7633, Vol. 8, no 2, 166-168 p.Article in journal (Refereed) Published
Abstract [en]

Chemical Equation Presented) Sugars in a row. A new strategy for carbohydrate microarrays based on photochemical ligation of perfluorophenylazide-derivatized carbohydrates to PEO surfaces is presented. It constitutes a controllable and robust method of array fabrication, on the carbohydrate chemistry and on the surface-chemistry levels, and the resulting carbohydrate arrays can be efficiently used to reveal the recognition patterns of carbohydrate-binding proteins.

Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 2007. Vol. 8, no 2, 166-168 p.
Keyword [en]
carbohydrates, lectins, microarrays, photochemistry, surfaces
National Category
Inorganic Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-6392DOI: 10.1002/cbic.200600447ISI: 000243810000003PubMedID: 17154195Scopus ID: 2-s2.0-33846914211OAI: oai:DiVA.org:kth-6392DiVA: diva2:11088
Note

QC 20100915

Available from: 2006-11-22 Created: 2006-11-22 Last updated: 2017-12-14Bibliographically approved
In thesis
1. Carbohydrate Synthesis and Study of Carbohydrate-Lectin Interactions Using QCM Biosensors and Microarray Technologies
Open this publication in new window or tab >>Carbohydrate Synthesis and Study of Carbohydrate-Lectin Interactions Using QCM Biosensors and Microarray Technologies
2006 (English)Doctoral thesis, comprehensive summary (Other scientific)
Abstract [en]

Interactions between carbohydrates and proteins are increasingly being recognized as crucial in many biological processes, such as cellular adhesion and communication. In order to investigate the interactions of carbohydrates and proteins, the development of efficient analytic technologies, as well as novel strategies for the synthesis of carbohydrates, have to be explored. To date, several methods have been exploited to analyze interactions of carbohydrates and proteins, for example, biosensors, nuclear magnetic resonance (NMR); enzyme-linked immunosorbent assays (ELISA), X-ray crystallography and array technologies. This thesis describes the development of novel strategies for the synthesis of carbohydrates, as well as new efficient strategies to Quartz Crystal Microbalance- (QCM-) biosensors and carbohydrate microarrays technologies. These methodologies have been used to probe carbohydrate-lectin-interactions for a range of plant and animal lectins.

Place, publisher, year, edition, pages
Stockholm: KTH, 2006. 106 p.
Series
Trita-IOK, ISSN 1100-7974 ; 2006:106
Keyword
Lectins; Carbohydrates; Molecular recognition; Biosensors; Quartz crystal microbalance; Carbohydrate microarrays.
National Category
Organic Chemistry
Identifiers
urn:nbn:se:kth:diva-4177 (URN)91-7178-503-5 (ISBN)
Public defence
2006-12-01, D3, Lindstedtsvägen 5, Stockholm, 10:00
Opponent
Supervisors
Note
QC 20100915Available from: 2006-11-22 Created: 2006-11-22 Last updated: 2011-09-05Bibliographically approved

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Pei, ZhichaoYu, HuiTheurer, MatthiasWaldén, AnnelieNilsson, PeterYan, MingdiRamström, Olof
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