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Multi-level characterization of balanced inhibitory-excitatory cortical neuron network derived from human pluripotent stem cells
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2017 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 12, no 6, 0178533Article in journal (Refereed) Published
Abstract [en]

Generation of neuronal cultures from induced pluripotent stem cells (hiPSCs) serve the studies of human brain disorders. However we lack neuronal networks with balanced excitatory- inhibitory activities, which are suitable for single cell analysis. We generated low-density networks of hPSC-derived GABAergic and glutamatergic cortical neurons. We used two different co-culture models with astrocytes. We show that these cultures have balanced excitatory-inhibitory synaptic identities using confocal microscopy, electrophysiological recordings, calcium imaging and mRNA analysis. These simple and robust protocols offer the opportunity for single-cell to multi-level analysis of patient hiPSC-derived cortical excitatory- inhibitory networks; thereby creating advanced tools to study disease mechanisms underlying neurodevelopmental disorders.

Place, publisher, year, edition, pages
Public Library of Science , 2017. Vol. 12, no 6, 0178533
Keyword [en]
Ganglionic Eminence, Astrocyte Coculture, Differentiation, Interneurons, Brain, Gaba, Specification, Maturation, Glutamate, Disease
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-209602DOI: 10.1371/journal.pone.0178533ISI: 000402875700018Scopus ID: 2-s2.0-85020237909OAI: oai:DiVA.org:kth-209602DiVA: diva2:1113218
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscienceEU, FP7, Seventh Framework Programme, FP7-PEOPLE-2013-ITN 607508
Note

QC 20170621

Available from: 2017-06-21 Created: 2017-06-21 Last updated: 2017-06-30Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
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More styles
Language
  • de-DE
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  • en-US
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  • nn-NB
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  • Other locale
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Output format
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  • asciidoc
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