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The lateral distance between a proton pump and ATP synthase determines the ATP-synthesis rate
KTH, School of Engineering Sciences (SCI), Applied Physics, Experimental Biomolecular Physics.
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2017 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 7, no 1, 2926Article in journal (Refereed) Published
Abstract [en]

We have investigated the effect of lipid composition on interactions between cytochrome bo 3 and ATP-synthase, and the ATP-synthesis activity driven by proton pumping. The two proteins were labeled by fluorescent probes and co-reconstituted in large (d ≅ 100 nm) or giant (d ≅ 10 μm) unilamellar lipid vesicles. Interactions were investigated using fluorescence correlation/cross-correlation spectroscopy and the activity was determined by measuring ATP production, driven by electron-proton transfer, as a function of time. We found that conditions that promoted direct interactions between the two proteins in the membrane (higher fraction DOPC lipids or labeling by hydrophobic molecules) correlated with an increased activity. These data indicate that the ATP-synthesis rate increases with decreasing distance between cytochrome bo 3 and the ATP-synthase, and involves proton transfer along the membrane surface. The maximum distance for lateral proton transfer along the surface was found to be ∼80 nm.

Place, publisher, year, edition, pages
Nature Publishing Group, 2017. Vol. 7, no 1, 2926
National Category
Biophysics
Identifiers
URN: urn:nbn:se:kth:diva-209856DOI: 10.1038/s41598-017-02836-4ISI: 000402879200011Scopus ID: 2-s2.0-85020432705OAI: oai:DiVA.org:kth-209856DiVA: diva2:1115920
Funder
Knut and Alice Wallenberg FoundationSwedish Research Council
Note

QC 20170627

Available from: 2017-06-27 Created: 2017-06-27 Last updated: 2017-07-03Bibliographically approved

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CiteExportLink to record
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