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Stimulated Emission Depletion Microscopy
KTH, School of Engineering Sciences (SCI), Applied Physics, Cellular Biophysics. KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, School of Engineering Sciences (SCI), Applied Physics, Experimental Biomolecular Physics.
2017 (English)In: Chemical Reviews, ISSN 0009-2665, E-ISSN 1520-6890, Vol. 117, no 11, 7377-7427 p.Article, review/survey (Refereed) Published
Abstract [en]

Despite its short history, diffraction-unlimited fluorescence microscopy techniques have already made a substantial imprint in the biological sciences. In this review, we describe how stimulated emission depletion (STED) imaging originally evolved, how it compares to other optical super-resolution imaging techniques, and what advantages it provides compared to previous golden-standards for biological microscopy, such as diffraction-limited optical microscopy and electron microscopy. We outline the prerequisites for successful STED imaging experiments, emphasizing the equally critical roles of instrumentation, sample preparation, and photophysics, and describe major evolving strategies for how to push the borders of STED imaging even further in life science. Finally, we provide examples of how STED nanoscopy can be applied, within three different fields with particular potential for STED imaging experiments: neuroscience, plasma membrane biophysics, and subcellular clinical diagnostics. In these areas, and in many more, STED imaging can be expected to play an increasingly important role in the future.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2017. Vol. 117, no 11, 7377-7427 p.
National Category
Biophysics
Identifiers
URN: urn:nbn:se:kth:diva-210275DOI: 10.1021/acs.chemrev.6b00653ISI: 000403631500005Scopus ID: 2-s2.0-85020766650OAI: oai:DiVA.org:kth-210275DiVA: diva2:1118230
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscienceSwedish Research Council, 2013-6041, 2011-3045EU, FP7, Seventh Framework Programme, FLUODIAMON 201 837Knut and Alice Wallenberg Foundation, 2011.0218Swedish Foundation for Strategic Research , RFI14-0091
Note

QC 20170630

Available from: 2017-06-30 Created: 2017-06-30 Last updated: 2017-07-11Bibliographically approved

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Blom, HansWidengren, Jerker
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CiteExportLink to record
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Citation style
  • apa
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