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Cytotoxic effect of a mannogalactoglucan extracted from Agaricus bisporus on HepG2 cells
KTH, School of Biotechnology (BIO), Glycoscience. Univ Fed Parana, Brazil.ORCID iD: 0000-0002-8533-1022
2017 (English)In: Carbohydrate Polymers, ISSN 0144-8617, E-ISSN 1879-1344, Vol. 170, 33-42 p.Article in journal (Refereed) Published
Abstract [en]

A mannogalactoglucan (RK2-Ab; M-w, 1.8 x 10(4) g mol(-1)) composed by Man (27.3%), Gal (24.4%) and Glc (48.3%) was extracted and characterized from Agaricus bisporus, and its biological activity was evaluated on human hepatocarcinoma cells (HepG2). The partially-O-methylated alditol acetates together with the NMR data suggest the main chain to be composed of alpha-D-Galp (32.8%) and beta-D-Glcp (37.0%) units (1 -> 6) -linked, with beta-D-Manp (14.6%), as non-reducing end units, substituting the side chains at O-2 (alpha-D-Galp units; 3.3%) and O-2 and O-4 (beta-D-Glcp units; 3.6%). (1 -> 2) -linked beta-D-Glcp (2.7%) and beta-D-Manp (6.0%) can also be observed. RK2-Ab reduced cellular viability of HepG2 cells, by both, the MTT and lactate dehydrogenase release assays, promoted the increase of cytochrome c release and decrease of ATP content. Suggesting that the mannogalactoglucan from A. bisporus may have antitumor activity by inducing apoptosis by the mitochondria death pathway, and could be used in cancer therapy.

Place, publisher, year, edition, pages
ELSEVIER SCI LTD , 2017. Vol. 170, 33-42 p.
Keyword [en]
Agaricus bisporus, Heteropolysaccharide, Mannogalactoglucan, Cytotoxicity, HepG2 cells
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-210450DOI: 10.1016/j.carbpol.2017.04.050ISI: 000402942900004PubMedID: 28522001Scopus ID: 2-s2.0-85018323534OAI: oai:DiVA.org:kth-210450DiVA: diva2:1120608
Note

QC 20170706

Available from: 2017-07-06 Created: 2017-07-06 Last updated: 2017-07-06Bibliographically approved

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