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High performance micro-flow cytometer based on optical fibres
KTH, School of Engineering Sciences (SCI), Applied Physics, Laser Physics. RISE Acreo AB, Sweden.
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0003-1176-0905
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0001-5199-0663
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
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2017 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 7, article id 5628Article in journal (Refereed) Published
Abstract [en]

Flow cytometry is currently the gold standard for analysis of cells in the medical laboratory and biomedical research. Fuelled by the need of point-of-care diagnosis, a significant effort has been made to miniaturize and reduce cost of flow cytometers. However, despite recent advances, current microsystems remain less versatile and much slower than their large-scale counterparts. In this work, an all-silica fibre microflow cytometer is presented that measures fluorescence and scattering from particles and cells. It integrates cell transport in circular capillaries and light delivery by optical fibres. Single-stream cell focusing is performed by Elasto-inertial microfluidics to guarantee accurate and sensitive detection. The capability of this technique is extended to high flow rates (up to 800 mu l/min), enabling a throughput of 2500 particles/s. The robust, portable and low-cost system described here could be the basis for a point-of-care flow cytometer with a performance comparable to commercial systems.

Place, publisher, year, edition, pages
Nature Publishing Group, 2017. Vol. 7, article id 5628
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-211606DOI: 10.1038/s41598-017-05843-7ISI: 000405677200013Scopus ID: 2-s2.0-85025168074OAI: oai:DiVA.org:kth-211606DiVA, id: diva2:1131311
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscienceSwedish Research CouncilKnut and Alice Wallenberg FoundationSwedish Childhood Cancer Foundation
Note

QC 20170814

Available from: 2017-08-14 Created: 2017-08-14 Last updated: 2017-10-17Bibliographically approved
In thesis
1. Advanced all-fiber optofluidic devices
Open this publication in new window or tab >>Advanced all-fiber optofluidic devices
2017 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Significant technological advances of the last years have been possible by developments in Optofluidics, which is a field that deals with the integration of optics and microfluidics into single devices.

The work described in this thesis is based on five scientific publications related to the use of fiber optic technology to build integrated optofluidic devices. The first three publications are within the field of life-science and point towards in-vivo and point-of-care applications, whereas the last two publications cover the study and the use of plasmonic nanoparticles for electrical modulation of light.

Aiming at developing useful tools for in-vivo biological applications, the first publication consists of designing and testing a functional optical fiber for real-time monitoring and selective collection of fluorescent microparticles. This probe relies on a microstructured optical fiber with a hole along its cladding, which is used to selectively aspirate individual particles of interest once their fluorescence signal is detected. On the same line of research, the second publication contemplates the fabrication of a fiber probe that traps single microparticles and allows for remote detection of their optical properties. This probe is also based on a microstructured fiber that enables particle trapping by fluidic forces. The third publication addresses the development of an all-fiber miniaturized flow cytometer for point-of-care applications. This system can analyze, with excellent accuracy and sensitivity, up to 2500 cells per second by measuring their fluorescence and scattering signal. A novel microfluidic technique, called Elasto-inertial microfluidics, is employed for aligning the cells into a single-stream to optimize detection and throughput.

The fourth publication involves the experimental and theoretical study of the electrical-induced alignment of plasmonic gold nanorods in suspension and its applicability to control light transmission. This study is done by using an all-fiber optofluidic device, based on a liquid-core fiber, which facilitates the interaction of light, electric fields, and liquid suspensions. Results show that nanorods can be aligned in microseconds, providing a much better performance than liquid-crystal devices. Finally, the fifth publication consists of an upgrade of the previous device by integrating four electrodes in the cladding of the liquid-core fiber. This improvement enables nanosecond response time and the possibility of digitally switching nanorods between two orthogonal aligned states, overcoming the limitation of slow thermal relaxation.

The work presented here shows that optofluidics based on optical fibers is a robust and convenient platform, as well as a promising direction for the developing of novel instruments in fields such as life-science, non-linear optics, plasmonic, and sensing.

Place, publisher, year, edition, pages
KTH Royal Institute of Technology, 2017. p. 68
Series
TRITA-FYS, ISSN 0280-316X ; 2017:65
Keywords
Fiber optics, functional fiber probes, optofluidics, microfluidics, plasmonic, all-fiber technology, instrumentation for life-sciences.
National Category
Medical Engineering Physical Sciences Medical Laboratory and Measurements Technologies
Research subject
Physics
Identifiers
urn:nbn:se:kth:diva-215938 (URN)978-91-7729-572-3 (ISBN)
Public defence
2017-11-14, FB42, Albanova, Roslagstullsbacken 21, KTH, Stockholm, 13:00 (English)
Opponent
Supervisors
Note

QC 20171018

Available from: 2017-10-18 Created: 2017-10-17 Last updated: 2017-10-18Bibliographically approved

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Etcheverry, SebastiánFaridi, Muhammad AsimRamachandraiah, HarishaMargulis, WalterLaurell, FredrikRussom, Aman

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