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A lateral flow cardiac troponin-T assay with colorimetric signal enhancement
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. (Nanobioteknologi)ORCID iD: 0000-0003-4856-860X
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. (Nanobioteknologi)
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. (Nanobioteknologi)
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. (Nanobioteknologi)
(English)Manuscript (preprint) (Other academic)
Abstract [en]

Cardiac troponin T (cTnT) is a biomarker for heart muscle damage such as in acute myocardial infarction (AMI). Its rapid assessment is needed to detect changes in the cTnT levels in blood for a quicker diagnosis of AMI. The sensitivity limit required to detect elevated levels of cTnT is 10 pg/mL, where the levels in the healthy population are 0.5-10 pg/mL. In this paper the detection of cardiac troponin T with a point-of-care lateral flow assay was investigated for the rapid diagnosis of AMI. It was studied by using different gold nanoparticle conjugation methods and colorimetric signal enhancement of detection gold nanoparticle conjugates could increase the sensitivity of a troponin T lateral flow microarray assay. The results indicate the same sensitivity range for the detection with gold nanoparticles functionalized with antibody by two different methods, and that the troponin T sandwich pair used might be essential for achieving a higher sensitivity. The signal enhancement increased the intensity signal of the detected cTnT on the array. The limit of detection of the assay changed from 10 μg/mL to 1 μg/mL for one conjugation method after signal enhancement but remained unchanged at 1 μg/mL for the other method.

National Category
Medical Biotechnology
Research subject
Biotechnology
Identifiers
URN: urn:nbn:se:kth:diva-214071OAI: oai:DiVA.org:kth-214071DiVA, id: diva2:1140134
Funder
EU, European Research Council, 615458
Note

QC 20170913

Available from: 2017-09-11 Created: 2017-09-11 Last updated: 2017-09-13Bibliographically approved

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Lama, LaraDias, Jorge T.Gantelius, JesperAndersson Svahn, Helene
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CiteExportLink to record
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Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
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Output format
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