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Investigating the effect of cell substrate on cancer cell stiffness by optical tweezers
KTH, School of Engineering Sciences (SCI), Applied Physics.
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2017 (English)In: Journal of Biomechanics, ISSN 0021-9290, E-ISSN 1873-2380, Vol. 60, 266-269 p.Article in journal (Refereed) Published
Abstract [en]

The mechanical properties of cells are influenced by their microenvironment. Here we report cell stiffness alteration by changing the cell substrate stiffness for isolated cells and cells in contact with other cells. Polydimethylsiloxane (PDMS) is used to prepare soft substrates with three different stiffness values (173, 88 and 17 kPa respectively). Breast cancer cells lines, namely HBL-100, MCF-7 and MDA-MB-231 with different level of aggressiveness are cultured on these substrates and their local elasticity is investigated by vertical indentation of the cell membrane. Our preliminary results show an unforeseen behavior of the MDA-MB-231 cells. When cultured on glass substrate as isolated cells, they are less stiff than the other two types of cells, in agreement with the general statement that more aggressive and metastatic cells are softer. However, when connected to other cells the stiffness of MDA-MB-231 cells becomes similar to the other two cell lines. Moreover, the stiffness of MDA-MB-231 cells cultured on soft PDMS substrates is significantly higher than the stiffness of the other cell types, demonstrating thus the strong influence of the environmental conditions on the mechanical properties of the cells. 

Place, publisher, year, edition, pages
ELSEVIER SCI LTD , 2017. Vol. 60, 266-269 p.
Keyword [en]
Cancer cell stiffness, Optical tweezers, Breast cancer, PDMS cell substrate, Cell microenvironment
National Category
Medical Engineering
Identifiers
URN: urn:nbn:se:kth:diva-214349DOI: 10.1016/j.jbiomech.2017.06.043ISI: 000408287300037PubMedID: 28712542Scopus ID: 2-s2.0-85023647891OAI: oai:DiVA.org:kth-214349DiVA: diva2:1140373
Note

QC 20170912

Available from: 2017-09-12 Created: 2017-09-12 Last updated: 2017-09-12Bibliographically approved

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CiteExportLink to record
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  • apa
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