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Developmental lignocellulose mapping in crop stems
KTH, School of Chemical Science and Engineering (CHE).
2017 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesisAlternative title
Kartläggning av lignocellulosa i växtstam (Swedish)
Abstract [en]

With current techniques there is no rapid, one-step alternative to investigate plant composition and anatomy. In this study we present the use of luminescent

conjugated oligothiophene (LCO), as a carbohydrate marker in unused cash crop stems. Stem samples were analyzed between day 0-75 using FTIR, gas

chromatography, confocal laser scanning microscopy and spectral imaging analysis. Carbohydrate composition analysis confirmed cellulose presence in all

time points tested and that fractions of monosaccharaides stay constant in the stem during growth. Using confocal and fluorescence microscopy, emission from

LCO bound to cellulose was detected and stem composition and anatomy visualized.

Overall, we demonstrate that LCOs can be utilized for compositional analyzes in plant tissues as a non-destructive and one-step alternative to today’s techniques.

This method shows high applicability for structure and anatomy studies in plant tissues, but also as a compliment for current composition analysis techniques.

 

Abstract [sv]

Med dagens tekniker finns det inget snabbt, enkelt alternativ för att undersöka komposition och anatomi hos plantor. I den här studien presenterar vi

användningen av luminescent conjugated oligothiophenes (LCO), som en kolhydratsmarkör i växtstjälkar. Stjälkprover analyserades mellan dag 0-75 med

FTIR, gaskromatografi, konfokal mikroskopi och spektral bildanalys. Analys av kolhydratskomposition bekräftade att cellulosa finns närvarande i alla prover

och att andelen av olika monosackarider är konstant i stjälken under dess tillväxt. Emission från LCO bunden till cellulosa detekterades och komposition

och anatomi av stjälken visualiserades med hjälp av konfokal mikroskopi och fluorescensmikroskopi.

Vi visar att LCOs kan utnyttjas för kompositionsanalys i plantvävnad som ett icke-destruktivt och enkelt alternativ till dagens tekniker. Denna metod visar

stor användbarhet för att studera struktur och anatomi i växtvävnader, men också som ett komplement till dagens analysmetoder för kompositionsanalys.

Place, publisher, year, edition, pages
2017. , p. 33
Keywords [en]
Cellulose, Cellulose detection, Carbohydrate composition, Fluorescence spectroscopy, Optical imaging, LCO
Keywords [sv]
Cellulosa, cellulosadetektion, kolhydratsammansättning, fluorescens-spektroskopi, LCO
National Category
Polymer Technologies
Identifiers
URN: urn:nbn:se:kth:diva-214987OAI: oai:DiVA.org:kth-214987DiVA, id: diva2:1145272
Available from: 2017-09-28 Created: 2017-09-28 Last updated: 2017-09-29Bibliographically approved

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