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Evaluation of ethylammonium nitrate as background electrolyte in capillary electrophoresis
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Applied Physical Chemistry. (Analytisk kemi)
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Applied Physical Chemistry.
KTH, School of Chemical Science and Engineering (CHE), Chemistry, Applied Physical Chemistry. KTH, Superseded Departments (pre-2005), Chemistry.ORCID iD: 0000-0002-3444-9987
(English)Manuscript (preprint) (Other academic)
National Category
Analytical Chemistry
Research subject
Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-215628OAI: oai:DiVA.org:kth-215628DiVA, id: diva2:1148463
Note

QC 20171013

Available from: 2017-10-11 Created: 2017-10-11 Last updated: 2017-10-13Bibliographically approved
In thesis
1. Bioanalytical separation using capillary electrophoresis: Applications with microbubbles and proteins
Open this publication in new window or tab >>Bioanalytical separation using capillary electrophoresis: Applications with microbubbles and proteins
2017 (English)Licentiate thesis, comprehensive summary (Other academic)
Abstract [en]

In this thesis the possibilities of using capillary electrophoresis as a separation technique for analysis of proteins and microbubbles is presented.

A complete analytical process consists of five necessary steps of which one is the actual analysis step. For this step a suitable analytical technique is needed. Capillary electrophoresis (CE) is one of the common analytical separation techniques used for analysis of a diversity of analytes, and can be both used in routine analysis and for research purposes. The reason for using CE, compared to other liquid-based separation techniques, is mainly short analysis time, high resolution, and negligible sample volumes and solvent waste. Depending on the characteristics of the analytes, and the sample matrix, different modes of CE can be used, where capillary zone electrophoresis (CZE) is the most employed one. The basic principle of CZE is separation of the analytes due to differences in total mobility, which is dependent on the charge and size of the analytes, and the electroosmotic flow (EOF). The EOF can be controlled by several parameters e.g. choice of background electrolyte (BGE), and the optimization of the parameters has been discussed throughout the thesis.

To improve the properties of the BGE, an ethylammonium nitrate (EAN) water solution was used as BGE for CE analysis in Paper I. The precision of the EOF with this method was determined by adjusting the pH of the BGE, the concentration of EAN in the BGE, and the electric field. Model proteins were thereafter analysed using the optimal parameters yielding a precision sufficient for routine control.

One example of the applications of CE is separation of novel contrast agents, which consist of polyvinyl alcohol microbubbles (PVA-MBs). In Paper II, a method for analysis of PVA-MBs in biological samples using CE with UV-detection was developed. It was also established that intact PVA-MBs could be distinguished from ultrasound degraded PVA-MBs in the same set-up.

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2017
Series
TRITA-CHE-Report, ISSN 1654-1081 ; 2017:32
Keywords
Background electrolyte, capillary electrophoresis, contrast agents, electroosmotic flow, ethylammonium nitrate, ionic liquids, microbubbles, particles, polyvinyl alcohol microbubbles, and separation
National Category
Analytical Chemistry
Research subject
Chemistry
Identifiers
urn:nbn:se:kth:diva-215194 (URN)978-91-7729-560-0 (ISBN)
Presentation
2017-11-23, K52, Teknikringen 28, plan 5, Stockholm, 10:00 (English)
Supervisors
Note

QC 20171012

Available from: 2017-10-12 Created: 2017-10-11 Last updated: 2017-10-12Bibliographically approved

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Emmer, Åsa

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