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Silk-Silk Interactions between Silkworm Fibroin and Recombinant Spider Silk Fusion Proteins Enable the Construction of Bioactive Materials
KTH, School of Biotechnology (BIO), Centres, Albanova VinnExcellence Center for Protein Technology, ProNova.
KTH, School of Biotechnology (BIO), Centres, Albanova VinnExcellence Center for Protein Technology, ProNova.
KTH, School of Biotechnology (BIO), Centres, Albanova VinnExcellence Center for Protein Technology, ProNova.
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2017 (English)In: ACS Applied Materials and Interfaces, ISSN 1944-8244, E-ISSN 1944-8252, Vol. 9, no 37, 31634-31644 p.Article in journal (Refereed) Published
Abstract [en]

Natural silk is easily accessible from silkworms and can be processed into different formats suitable as biomaterials and cell culture matrixes. Recombinant DNA technology enables chemical-free functionalization of partial silk proteins through fusion with peptide motifs and protein domains, but this constitutes a less cost-effective production process. Herein, we show that natural silk fibroin (SF) can be used as a bulk material that can be top-coated with a thin layer of the recombinant spider silk protein 4RepCT in fusion with various bioactive motifs and domains. The coating process is based on a silk assembly to achieve stable interactions between the silk types under mild buffer conditions. The assembly process was studied in real time by quartz crystal microbalance with dissipation. Coatings, electrospun mats, and microporous scaffolds were constructed from Antheraea assama and Bombyx mori SFs. The morphology of the fibroin materials before and after coating with recombinant silk proteins was analyzed by scanning electron microscopy and atomic force microscopy. SF materials coated with various bioactive 4RepCT fusion proteins resulted in directed antibody capture, enzymatic activity, and improved cell attachment and spreading, respectively, compared to pristine SF materials. The herein-described procedure allows a fast and easy route for the construction of bioactive materials.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC , 2017. Vol. 9, no 37, 31634-31644 p.
Keyword [en]
biomaterial, silk fibroin, recombinant spider silk, functionalization, self-assembly
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:kth:diva-215821DOI: 10.1021/acsami.7b10874ISI: 000411771400044PubMedID: 28846369Scopus ID: 2-s2.0-85029739918OAI: oai:DiVA.org:kth-215821DiVA: diva2:1149923
Note

QC 20171017

Available from: 2017-10-17 Created: 2017-10-17 Last updated: 2017-10-17Bibliographically approved

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Hedhammar, My

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Nilebäck, LinneaJansson, RonnieWidhe, MonaHedhammar, My
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