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Neuroproteomic profiling of cerebrospinal fluid (CSF) by multiplexed affinity arrays
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab. (Affinity Proteomics)
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab. (Affinity Proteomics)
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab. (Affinity Proteomics)ORCID iD: 0000-0001-8141-8449
2017 (English)In: Neuroproteomics: Methods and Protocols, Humana Press, 2017, p. 247-254Chapter in book (Refereed)
Abstract [en]

Protein profiling through affinity proteomic approaches represents a powerful strategy for the analysis of human body fluids. Cerebrospinal fluid (CSF), being the fluid proximal to the central nervous system, is commonly analyzed in the context of neurological diseases, and can offer novel insights into the physiological state of the brain. Ultimately, and by analyzing the presence of brain-derived proteins in larger sets of samples that represent different phenotypes, profiling of CSF may serve as an important source to discover and verify disease-associated markers. Here, we describe a multiplexed and flexible protein profiling approach using antibody-based assays on suspension bead arrays. Through streamlined sample processing, protein biotinylation, and single-binder assay readout, this method enables high-throughput neuroproteomic analysis of up to 384 proteins in 384 samples.

Place, publisher, year, edition, pages
Humana Press, 2017. p. 247-254
Series
Methods in Molecular Biology, ISSN 1064-3745 ; 1598
Keywords [en]
Antibody, Cerebrospinal fluid, Direct labeling, Microarray, Protein profiling, Suspension bead array
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-216349DOI: 10.1007/978-1-4939-6952-4_11Scopus ID: 2-s2.0-85019567527ISBN: 978-1-4939-6950-0 (print)OAI: oai:DiVA.org:kth-216349DiVA, id: diva2:1150631
Funder
VINNOVAKnut and Alice Wallenberg FoundationScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20171019

Available from: 2017-10-19 Created: 2017-10-19 Last updated: 2017-10-19Bibliographically approved

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