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High-density serum/plasma reverse phase protein arrays
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab. (Affinity Proteomics)
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab. (Affinity Proteomics)
KTH, Centres, Science for Life Laboratory, SciLifeLab. (Affinity Proteomics)
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab. (Affinity Proteomics)
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2017 (English)In: Serum/Plasma Proteomics, Humana Press, 2017, 229-238 p.Chapter in book (Refereed)
Abstract [en]

In-depth exploration and characterization of human serum and plasma proteomes is an attractive strategy for the identification of potential prognostic or diagnostic biomarkers. The possibility of analyzing larger numbers of samples in a high-throughput fashion has markedly increased with affinity-based microarrays, thus providing higher statistical power to these biomarker studies. Here, we describe a protocol for high-density serum and plasma reverse phase protein arrays (RPPAs). We demonstrate how a biobank of 12,392 samples was immobilized and analyzed on a single microarray slide, allowing high-quality profiling of abundant target proteins across all samples in one assay.

Place, publisher, year, edition, pages
Humana Press, 2017. 229-238 p.
Series
Methods in Molecular Biology, ISSN 1064-3745 ; 1619
Keyword [en]
Affinity proteomics, Fluorescent detection, Noncontact inkjet printer, Plasma, Protein profiling, Reverse phase protein array, RPPA, Serum
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-216337DOI: 10.1007/978-1-4939-7057-5_18Scopus ID: 2-s2.0-85021766648OAI: oai:DiVA.org:kth-216337DiVA: diva2:1150980
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscienceKnut and Alice Wallenberg Foundation
Note

QC 20171020

Available from: 2017-10-20 Created: 2017-10-20 Last updated: 2017-10-20Bibliographically approved

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Hellström, CeciliaDodig-Crnković, TeaHong, Mun-GwanSchwenk, JochenNilsson, PeterSjöberg, Ronald
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Proteomics and NanobiotechnologyScience for Life Laboratory, SciLifeLab
Analytical Chemistry

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