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A Probe for the Detection of Hypoxic Cancer Cells
KTH, School of Biotechnology (BIO), Theoretical Chemistry and Biology.
2017 (English)In: ACS SENSORS, ISSN 2379-3694, Vol. 2, no 8, 1139-1145 p.Article in journal (Refereed) Published
Abstract [en]

Hypoxia is a common feature of tumor cells. Nitroreductase (NTR), a common biomarker of hypoxia, has been widely used to evaluate the extent of tumor hypoxia. In this study, three fluorescent probes (FBN-1-3) were synthesized to monitor the extent of hypoxia in cancer cells in real time. FBN-1-3 were composed of a fluorescein analogue and one of three different aromatic nitro groups. Of these probes, FBN-1 showed excellent sensitivity and selectivity in detecting hypoxia via a reduction in O-2 concentration. Confocal fluorescence imaging and flow cytometry demonstrated that HepG-2, A549, and SKOV-3 cells incubated with FBN-1 under reduced oxygen conditions showed significantly enhanced fluorescence. A mouse HepG-2 tumor model confirmed that FBN-1 responds rapidly to NTR and can be used to evaluate the degree of tumor hypoxia. The changes in intra- and extracellular NTR in tumor cells were also concurrently monitored, which did not reveal a link between NTR concentration and degree of hypoxia. Our work provides a functional probe for tumor hypoxia, and our results suggest the fluorescent response of our probe is due to a decrease in O-2 concentration, and not NTR concentration.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC , 2017. Vol. 2, no 8, 1139-1145 p.
Keyword [en]
fluorescent probes, nitroreductase, hypoxic fluorescent imaging, tumor detection, hypoxia sensors
National Category
Nano Technology
Identifiers
URN: urn:nbn:se:kth:diva-214899DOI: 10.1021/acssensors.7b00171ISI: 000408702500008PubMedID: 28741347Scopus ID: 2-s2.0-85028306650OAI: oai:DiVA.org:kth-214899DiVA: diva2:1151251
Note

QC 20171023

Available from: 2017-10-23 Created: 2017-10-23 Last updated: 2017-10-23Bibliographically approved

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Zou, Rongfeng

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CiteExportLink to record
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Citation style
  • apa
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  • modern-language-association-8th-edition
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