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Formulation of Delivery System for Carotenoid-Rich Extracts from Microalgae
KTH, School of Chemical Science and Engineering (CHE).
2017 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesisAlternative title
Formulering av bäraresystem för carotenoid-rik extrakt från mikroalgae (Swedish)
Abstract [en]

This thesis is a part of the D-factory project, which aims to develop a sustainable bio refinery from the carotenoid-rich microalgae Dunaliella salina.

An extract, containing approximately 30% carotenoids where the majority is -carotene, from the microalgae has been incorporated into o/w nanoemulsions and suspensions of nanostructured lipid carriers (NLC) , which are supposed to be administrated orally and topically respectively. The nanoemulsions are to be added as a nutritional concentrate in a smoothie, where one dose (approximately 3-SmL) contains a daily intake of -carotene with respect to its pro vitamin A activity, while the NLC suspensions are to be used in a treating skin cream.

2.5% (w/w) extract was successfully incorporated in the oil phase of nanoemulsions, based on medium chain triglycer ides, MCT, in water with Lipoid LPC as emuls ifie r, that were physically stable at room temperature for over 4 weeks storage time . Two different emulsions  with  different oil compositions were chosen; one containing 10% (w/ w) oil and  one that contained 40% (w/w) oil. Nanostructured lipid carriers with a lipid matrix based on stearic acid and MCT and also carnauba wax and MCT, both with 2.5% (w/w) extract in the lipid phase, that were  stable  for more than 4 weeks of storage at  room temperature  were also developed.

Besides the formulation of the mentioned delivery systems, their stability  has  also  been evaluated, both with regards to their physical stability and also their ability to preserve the carotenoids in the extract from degradation, over  time  at  three  different  storage  conditions; room temperature, UV-exposure at room temperature and 40°C (protected from UV-light). No significant differences in particle size were seen in any of the delivery systems at room temperature, however a significant increase in particle size was observed for the nanostructured lipid carrier suspensions at 40°C. Out of the four delivery systems evaluated, the emulsion containing 40% (w/w) oil showed the best chemical stability at all storage conditions. The worst stability was noted at the suspensions, which is not in agreement with previous findings  that have shown  the opposite.

 

 

Abstract [sv]

Det här examensarbetet är en del av projektet "D-factory", som syftar till att utveckla ett hållbart bioraffinaderi för den karotenoidrika mikroalgen Dunaliella salina .

Ett extrakt, innehållandes ungefär 30 % karotenoider där merparten är betakaroten, från mikroalgen har inkorporerats o/w nanoemulsioner och suspensioner  av  fasta lipidnanopartiklar, med syftet att i en tänkt slutprodukt kunna administreras oralt respektive topiskt. Nanoemulsionerna skall kunna användas som ett tillägg till en smoothie, där en dos (ca 3-5mL) innehåller ett dagligt intag av betakaroten med avseende på dess pro A-vitamin­aktivitet, medan suspensionerna skall kunna användas i en vårdande hudkräm.

2,5 vikt% extrakt i oljefasen inkorporerades framgångsrikt  i emulsioner, baserade på medium chain triglycerides, MCT, i vatten med Lipoid LPC som tensid, som var fysiskt stabila i rumstemperatur i över 4 veckors tid.  Två olika emulsioner med olika oljekoncentration togs fram; 10 vikt% olja och 40 vikt% olja . Även fasta lipidnanopartikelsuspensioner baserade på stearinsyra och MCT samt carnaubavax och  MCT, båda med 2,5 vikt% extrakt i lipidfasen, som var stabila  i över 4 veckor vid rumstemperatur togs fram.

Utöver formuleringen av ovan nämnda bärsystem har stabiliteten hos dessa undersökts, både med avseende på dess fysiska stabilitet och på nedbrytningen av karotenoiderna i extraktet, över tid under tre lagringsförhållanden; rumstemperatur, UV-bestrålning vid  rumstemperatur och 40°C. Inga signifikanta skillnader i partikelstorlek uppmättes hos något av systemen vid rumstemperatur, dock ökade partikelstorleken signifikant för båda suspensionerna vid 40 °C. Av samtliga bärsystem visade emulsionen innehållandes 40 vikt% olja på bäst kemisk stabilitet vid alla lagringsförhållanden. Sämst stabilitet noterades hos suspensionerna, vilket inte stämmer överens med tidigare studier som visat på motsatt effekt.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Place, publisher, year, edition, pages
2017.
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:kth:diva-217241OAI: oai:DiVA.org:kth-217241DiVA, id: diva2:1154619
External cooperation
RISE
Available from: 2017-11-03 Created: 2017-11-03 Last updated: 2017-11-03Bibliographically approved

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