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Multifocus structured illumination microscopy for fast volumetric super-resolution imaging
KTH, School of Engineering Sciences (SCI), Applied Physics.ORCID iD: 0000-0002-5584-9170
KTH, School of Engineering Sciences (SCI), Applied Physics.
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2017 (English)In: Biomedical Optics Express, ISSN 2156-7085, E-ISSN 2156-7085, Vol. 8, no 9, p. 4135-4140, article id #294866Article in journal (Refereed) Published
Abstract [en]

We here report for the first time the synergistic implementation of structured illumination microscopy (SIM) and multifocus microscopy (MFM). This imaging modality is designed to alleviate the problem of insufficient volumetric acquisition speed in superresolution biological imaging. SIM is a wide-field super-resolution technique that allows imaging with visible light beyond the classical diffraction limit. Employing multifocus diffractive optics we obtain simultaneous wide-field 3D imaging capability in the SIM acquisition sequence, improving volumetric acquisition speed by an order of magnitude. Imaging performance is demonstrated on biological specimens.

Place, publisher, year, edition, pages
OSA - The Optical Society , 2017. Vol. 8, no 9, p. 4135-4140, article id #294866
Keywords [en]
Fluorescence microscopy, Imaging systems, Microscopy, Superresolution, Three-dimensional microscopy, Diffraction, Fluorescence, Image reconstruction, Light, Mergers and acquisitions, Microscopic examination, Optical resolving power, Biological specimens, Imaging performance, Structured illumination microscopies (SIM), Structured illumination microscopy, Super resolution, Super resolution imaging, Volumetric acquisitions, Magnetic force microscopy
National Category
Atom and Molecular Physics and Optics Medical Laboratory and Measurements Technologies
Identifiers
URN: urn:nbn:se:kth:diva-216180DOI: 10.1364/BOE.8.004135ISI: 000409020600017Scopus ID: 2-s2.0-85028755480OAI: oai:DiVA.org:kth-216180DiVA, id: diva2:1154877
Note

QC 20171106

Available from: 2017-11-06 Created: 2017-11-06 Last updated: 2017-11-06Bibliographically approved

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Blom, HansNilsson, LinneaBrismar, Hjalmar

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CiteExportLink to record
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  • apa
  • harvard1
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  • de-DE
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Output format
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