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Measuring enzyme kinetics of glycoside hydrolases using the 3,5-dinitrosalicylic acid assay
KTH, School of Biotechnology (BIO), Glycoscience.ORCID iD: 0000-0002-3372-8773
2017 (English)In: Methods in Molecular Biology, Humana Press Inc. , 2017, p. 27-36Conference paper (Refereed)
Abstract [en]

Use of the 3,5-dinitrosalicylic acid reagent allows the simple and rapid quantification of reducing sugars. The method can be used for analysis of biological samples or in the characterization of enzyme reactions. Presented here is an application of the method in measuring the kinetics of a glycoside hydrolase reaction, including the optimization of the DNSA reagent, and the production of a standard curve of absorbance and sugar concentration.

Place, publisher, year, edition, pages
Humana Press Inc. , 2017. p. 27-36
Keywords [en]
3, 5-dinitrosalicylic acid, Enzyme kinetics, Glycoside hydrolase, Reducing sugars, UV–visible spectrophotometry, 3, 5 dinitrosalicylic acid, glycosidase, reagent, salicylic acid derivative, sugar, unclassified drug, assay, enzyme mechanism, chemistry, enzyme assay, kinetics, metabolism, procedures, Enzyme Assays, Glycoside Hydrolases, Salicylates
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-216559DOI: 10.1007/978-1-4939-6899-2_3Scopus ID: 2-s2.0-85018584524OAI: oai:DiVA.org:kth-216559DiVA, id: diva2:1155659
Note

Funding details: Knut och Alice Wallenbergs Stiftelse; Funding details: KTH, Kungliga Tekniska Högskolan; Funding details: Svenska Forskningsrådet Formas; Funding text: The author thanks the support of the Knut and Alice Wallenberg Foundation through the Wallenberg Wood Science Centre, Sweden, as well as the KTH Carbohydrate Materials Consortium (CarboMat) supported by the Swedish Research Council FORMAS.

QC 20171108

Available from: 2017-11-08 Created: 2017-11-08 Last updated: 2017-11-08Bibliographically approved

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McKee, Lauren S.

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CiteExportLink to record
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Citation style
  • apa
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Output format
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