Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Achieving high-efficiency emission depletion nanoscopy by employing cross relaxation in upconversion nanoparticles
KTH, School of Biotechnology (BIO), Theoretical Chemistry and Biology.
Show others and affiliations
2017 (English)In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 8, no 1, article id 1058Article in journal (Refereed) Published
Abstract [en]

Stimulated emission depletion microscopy provides a powerful sub-diffraction imaging modality for life science studies. Conventionally, stimulated emission depletion requires a relatively high light intensity to obtain an adequate depletion efficiency through only light–matter interaction. Here we show efficient emission depletion for a class of lanthanide-doped upconversion nanoparticles with the assistance of interionic cross relaxation, which significantly lowers the laser intensity requirements of optical depletion. We demonstrate two-color super-resolution imaging using upconversion nanoparticles (resolution ~ 66 nm) with a single pair of excitation/depletion beams. In addition, we show super-resolution imaging of immunostained cytoskeleton structures of fixed cells (resolution ~ 82 nm) using upconversion nanoparticles. These achievements provide a new perspective for the development of photoswitchable luminescent probes and will broaden the applications of lanthanide-doped nanoparticles for sub-diffraction microscopic imaging.

Place, publisher, year, edition, pages
Nature Publishing Group, 2017. Vol. 8, no 1, article id 1058
Keywords [en]
Structured-Illumination Microscopy, Doped Silica Fibers, Sted Microscopy, Fluorescence Microscopy, Stimulated-Emission, Resolution Limit, Cation-Exchange, Quantum Dots, Luminescence, Laser
National Category
Atom and Molecular Physics and Optics
Identifiers
URN: urn:nbn:se:kth:diva-217389DOI: 10.1038/s41467-017-01141-yISI: 000413353500007PubMedID: 29051497Scopus ID: 2-s2.0-85031908749OAI: oai:DiVA.org:kth-217389DiVA, id: diva2:1156312
Funder
Swedish Research Council, 2015-00160;2016-03804
Note

QC 20171113

Available from: 2017-11-11 Created: 2017-11-11 Last updated: 2017-11-29Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Liu, HaichunÅgren, HansHe, Sailing
By organisation
Theoretical Chemistry and BiologyElectromagnetic Engineering
In the same journal
Nature Communications
Atom and Molecular Physics and Optics

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 92 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf