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Laboratory cryo x-ray microscopy for 3D cell imaging
KTH, School of Engineering Sciences (SCI), Applied Physics.
KTH, School of Engineering Sciences (SCI), Applied Physics, Biomedical and X-ray Physics.ORCID iD: 0000-0002-3717-7307
KTH, School of Engineering Sciences (SCI), Applied Physics, Cellular Biophysics.ORCID iD: 0000-0001-5178-7593
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2017 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 7, 13433Article in journal (Refereed) Published
Abstract [en]

Water-window x-ray microscopy allows two-and three-dimensional (2D and 3D) imaging of intact unstained cells in their cryofixed near-native state with unique contrast and high resolution. Present operational biological water-window microscopes are based at synchrotron facilities, which limits their accessibility and integration with complementary methods. Laboratory-source microscopes have had difficulty addressing relevant biological tasks with proper resolution and contrast due to long exposure times and limited up-time. Here we report on laboratory cryo x-ray microscopy with the exposure time, contrast, and reliability to allow for routine high-spatial resolution 3D imaging of intact cells and cell-cell interactions. Stabilization of the laser-plasma source combined with new optics and sample preparation provide high-resolution cell imaging, both in 2D with ten-second exposures and in 3D with twenty-minute tomography. Examples include monitoring of the distribution of carbon-dense vesicles in starving HEK293T cells and imaging the interaction between natural killer cells and target cells.

Place, publisher, year, edition, pages
NATURE PUBLISHING GROUP , 2017. Vol. 7, 13433
National Category
Physical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-217434DOI: 10.1038/s41598-017-13538-2ISI: 000413188400014PubMedID: 29044158Scopus ID: 2-s2.0-85031924153OAI: oai:DiVA.org:kth-217434DiVA: diva2:1158157
Note

QC 20171117

Available from: 2017-11-17 Created: 2017-11-17 Last updated: 2017-11-17Bibliographically approved

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Kördel, MikaelÖnfelt, BjörnHertz, Hans

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