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Slipdisc: A versatile sample preparation platform for point of care diagnostics
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0001-5199-0663
KTH, School of Chemical Science and Engineering (CHE), Fibre and Polymer Technology, Fibre Technology.ORCID iD: 0000-0003-1874-2187
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2017 (English)In: RSC Advances, ISSN 2046-2069, E-ISSN 2046-2069, Vol. 7, no 56, p. 35048-35054Article in journal (Refereed) Published
Abstract [en]

We report a microfluidic sample preparation platform called "Slipdisc" based on slipchip technology. Slipdisc is a rotational slipchip that uses a unique hand-wound clockwork mechanism for precise movement of specially fabricated polycarbonate discs. In operation, the microchannels and microchambers carved on the closely aligned microfluidic discs convert from continuous filled paths to defined compartments using the slip movement. The clockwork mechanism introduced here is characterised by a food dye experiment and a conventional HRP TMB reaction before measuring lactate dehydrogenase (LDH) enzyme levels, which is a crucial biomarker for neonatal diagnostics. The colorimetry based detection of LDH was performed with an unmodified camera and an image analysis procedure based on normalising images and observing changes in red channel intensity. The analysis showed a close to unity coefficient of determination (R2 = 0.96) in detecting the LDH concentration when compared with a standard Chemical Analyser, demonstrating the excellent performance of the slipdisc platform with colorimetric detection. The versatile point of care sample preparation platform should ideally be suited for a multitude of applications at resource-limited settings.

Place, publisher, year, edition, pages
Royal Society of Chemistry , 2017. Vol. 7, no 56, p. 35048-35054
Keywords [en]
Chemical analysis, Chemical detection, Clocks, Colorimetric analysis, Colorimetry, Coefficient of determination, Colorimetric detection, Lactate dehydrogenase, Micro-chambers, Point of care, Point of care diagnostic, Red channels, Sample preparation, Microfluidics
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-219077DOI: 10.1039/c7ra05209jISI: 000405811400015Scopus ID: 2-s2.0-85025080747OAI: oai:DiVA.org:kth-219077DiVA, id: diva2:1161966
Note

QC 20171201

Available from: 2017-12-01 Created: 2017-12-01 Last updated: 2018-02-15Bibliographically approved

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Banerjee, IndradumnaSalih, TagridRamachandraiah, HarishaErlandsson, JohanPettersson, TorbjörnRussom, Aman

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Banerjee, IndradumnaSalih, TagridRamachandraiah, HarishaErlandsson, JohanPettersson, TorbjörnRussom, Aman
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Proteomics and NanobiotechnologyScience for Life Laboratory, SciLifeLabFibre TechnologySurface and Corrosion ScienceWallenberg Wood Science CenterVinnExcellence Center BiMaC InnovationChemistry
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