Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A subcellular map of the human proteome
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0002-6107-1465
KTH, School of Biotechnology (BIO). KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0002-6368-6690
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
Show others and affiliations
2017 (English)In: Science, ISSN 0036-8075, E-ISSN 1095-9203, Vol. 356, no 6340, article id 820Article in journal (Refereed) Published
Abstract [en]

Resolving the spatial distribution of the human proteome at a subcellular level can greatly increase our understanding of human biology and disease. Here we present a comprehensive image-based map of subcellular protein distribution, the Cell Atlas, built by integrating transcriptomics and antibody-based immunofluorescence microscopy with validation by mass spectrometry. Mapping the in situ localization of 12,003 human proteins at a single-cell level to 30 subcellular structures enabled the definition of the proteomes of 13 major organelles. Exploration of the proteomes revealed single-cell variations in abundance or spatial distribution and localization of about half of the proteins to multiple compartments. This subcellular map can be used to refine existing protein-protein interaction networks and provides an important resource to deconvolute the highly complex architecture of the human cell.

Place, publisher, year, edition, pages
American Association for the Advancement of Science , 2017. Vol. 356, no 6340, article id 820
Keywords [en]
antibody, proteome, biology, cells and cell components, disease incidence, image analysis, physiological response, protein, proteomics, spatial distribution, Article, cell organelle, cellular distribution, human, human cell, immunofluorescence microscopy, mass spectrometry, priority journal, protein analysis, protein localization, protein protein interaction, single cell analysis, transcriptomics
National Category
Cell Biology
Identifiers
URN: urn:nbn:se:kth:diva-216588DOI: 10.1126/science.aal3321ISI: 000401957900032Scopus ID: 2-s2.0-85019201137OAI: oai:DiVA.org:kth-216588DiVA, id: diva2:1164056
Note

QC 20171208

Available from: 2017-12-08 Created: 2017-12-08 Last updated: 2017-12-08Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textScopus

Authority records BETA

Thul, Peter J.Wiking, MikaelaMahdessian, DianaAit Blal, HammouAlm, Tove L.Bäckström, AnnaDanielsson, FridaFagerberg, LinnFall, JennyGnann, ChristianHober, SophiaHjelmare, MartinLee, SunjaeNilsson, PeterOksvold, PerRockberg, JohanSchutten, RutgerSchwenk, Jochen M.Sivertsson, ÅsaSkogs, MarieStadler, CharlotteSullivan, Devin P.Tegel, HannaWinsnes, Casper F.Zwahlen, MartinMardinoglu, Adilvon Feilitzen, KalleUhlén, MathiasLundberg, Emma

Search in DiVA

By author/editor
Thul, Peter J.Åkesson, LovisaWiking, MikaelaMahdessian, DianaAit Blal, HammouAlm, Tove L.Björk, LarsBäckström, AnnaDanielsson, FridaFagerberg, LinnFall, JennyGnann, ChristianHober, SophiaHjelmare, MartinJohansson, FredricLee, SunjaeNilsson, PeterOksvold, PerRockberg, JohanSchutten, RutgerSchwenk, Jochen M.Sivertsson, ÅsaSkogs, MarieStadler, CharlotteSullivan, Devin P.Tegel, HannaWinsnes, Casper F.Zhang, ChengZwahlen, MartinMardinoglu, Adilvon Feilitzen, KalleUhlén, MathiasLundberg, Emma
By organisation
Proteomics and NanobiotechnologyScience for Life Laboratory, SciLifeLabSchool of Biotechnology (BIO)Protein Technology
In the same journal
Science
Cell Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 95 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf