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Plasmon-enhanced colorimetric ELISA with single molecule sensitivity.
2011 (English)In: Nano letters (Print), ISSN 1530-6984, E-ISSN 1530-6992, Vol. 11, no 4Article in journal (Refereed) Published
Abstract [en]

Robust but ultrasensitive biosensors with a capability of detecting low abundance biomarkers could revolutionize clinical diagnostics and enable early detection of cancer, neurological diseases, and infections. We utilized a combination of localized surface plasmon resonance (LSPR) refractive index sensing and the well-known enzyme-linked immunosorbent assay to develop a simple colorimetric biosensing methodology with single molecule sensitivity. The technique is based on spectral imaging of a large number of isolated gold nanoparticles. Each particle binds a variable number of horseradish peroxidase (HRP) enzyme molecules that catalyze a localized precipitation reaction at the particle surface. The enzymatic reaction dramatically amplifies the shift of the LSPR scattering maximum, λ(max), and makes it possible to detect the presence of only one or a few HRP molecules per particle.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2011. Vol. 11, no 4
National Category
Nano Technology Other Physics Topics Atom and Molecular Physics and Optics
Identifiers
URN: urn:nbn:se:kth:diva-197583DOI: 10.1021/nl2006092PubMedID: 21428275Scopus ID: 2-s2.0-79954500095OAI: oai:DiVA.org:kth-197583DiVA, id: diva2:1165971
Note

QC 20171218

Available from: 2017-12-14 Created: 2017-12-14 Last updated: 2017-12-18Bibliographically approved

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