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Streamlined Preparation of Immobilized Candida antarctica Lipase B
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2017 (English)In: ACS OMEGA, ISSN 2470-1343, Vol. 2, no 12, 8674-8677 p.Article in journal (Refereed) Published
Abstract [en]

Candida antarctica lipase B (CalB) was efficiently expressed (6.2 g L-1) in Escherichia coli by utilizing an N-terminal tag cassette and the XylS/Pm expression system in a fed-batch bioreactor; subsequent direct binding to EziG from crude extracts resulted in an immobilized catalyst with superior activity to Novozym 435.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2017. Vol. 2, no 12, 8674-8677 p.
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Chemical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-221018DOI: 10.1021/acsomega.7b01510ISI: 000418744400014OAI: oai:DiVA.org:kth-221018DiVA: diva2:1173459
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QC 20180112

Available from: 2018-01-12 Created: 2018-01-12 Last updated: 2018-01-12Bibliographically approved

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