A comparison between Dual Polarization Interferometry (DPI) and Surface Plasmon Resonance (SPR) for protein adsorption studies
2007 (English)In: Colloids and Surfaces B: Biointerfaces, ISSN 0927-7765, E-ISSN 1873-4367, Vol. 54, no 2, 236-240 p.Article in journal (Refereed) Published
This work was performed with the aim of comparing protein adsorption results obtained from the recently developed dual polarization interferometry (DPI) with the well-established surface plasmon resonance (SPR) technique. Both techniques use an evanescent field as the sensing element but completely different methods to calculate the adsorbed mass. As a test system we used adsorption of the lipase from Thermomyces lanuginosus (TLL) on C18 surfaces. The adsorbed amount calculated with both techniques is in good agreement, with both adsorption isotherms saturating at 1.30-1.35 mg/m(2) at TLL concentrations of 1000 nM and above. Therefore, this supports the use of both SPR and DPI as tools for studying protein adsorption, which is very important when comparing adsorption data obtained from the use different techniques. Due to the spot sensing in SPR, this technique is recommended for initial kinetic studies, whereas DPI is more accurate when the refractive index and thickness of the adsorbed layer is of more interest.
Place, publisher, year, edition, pages
2007. Vol. 54, no 2, 236-240 p.
C18-surface; Dual polarization interferometry; Lipase; Protein adsorption; Surface plasmon resonance; Adsorption; Adsorption isotherms; Enzymes; Interferometry; Reaction kinetics; Refractive index; Surface plasmon resonance; triacylglycerol lipase; adsorption; adsorption chromatography; article; calculation; chemical reaction kinetics; interferometry; intermethod comparison; nonhuman; priority journal; surface plasmon resonance; Adsorption; Ascomycota; Interferometry; Lipase; Mitosporic Fungi; Proteins; Surface Plasmon Resonance
IdentifiersURN: urn:nbn:se:kth:diva-6927DOI: 10.1016/j.colsurfb.2006.10.028ISI: 000244473900016ScopusID: 2-s2.0-33846372015OAI: oai:DiVA.org:kth-6927DiVA: diva2:11778
QC 201008182007-03-272007-03-272010-08-18Bibliographically approved