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Platelet protein biomarker panel for ovarian cancer diagnosis
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2018 (English)In: BIOMARKER RESEARCH, ISSN 2050-7771, Vol. 6, article id 2Article in journal (Refereed) Published
Abstract [en]

Background: Platelets support cancer growth and spread making platelet proteins candidates in the search for biomarkers. Methods: Two-dimensional (2D) gel electrophoresis, Partial Least Squares Discriminant Analysis (PLS-DA), Western blot, DigiWest. Results: PLS-DA of platelet protein expression in 2D gels suggested differences between the International Federation of Gynaecology and Obstetrics (FIGO) stages III-IV of ovarian cancer, compared to benign adnexal lesions with a sensitivity of 96% and a specificity of 88%. A PLS-DA-based model correctly predicted 7 out of 8 cases of FIGO stages I-II of ovarian cancer after verification by western blot. Receiver-operator curve (ROC) analysis indicated a sensitivity of 83% and specificity of 76% at cut-off >0.5 (area under the curve (AUC) = 0.831, p < 0.0001) for detecting these cases. Validation on an independent set of samples by DigiWest with PLS-DA differentiated benign adnexal lesions and ovarian cancer, FIGO stages III-IV, with a sensitivity of 70% and a specificity of 83%. Conclusion: We identified a group of platelet protein biomarker candidates that can quantify the differential expression between ovarian cancer cases as compared to benign adnexal lesions.

Place, publisher, year, edition, pages
BIOMED CENTRAL LTD , 2018. Vol. 6, article id 2
Keywords [en]
Ovarian cancer, Platelet proteome, Biomarker, Liquid biopsy
National Category
Medical Biotechnology
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URN: urn:nbn:se:kth:diva-221935DOI: 10.1186/s40364-018-0118-yISI: 000419918200001PubMedID: 29344361OAI: oai:DiVA.org:kth-221935DiVA, id: diva2:1178892
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QC 20180131

Available from: 2018-01-31 Created: 2018-01-31 Last updated: 2018-01-31Bibliographically approved

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Bergstrand, JanWidengren, Jerker

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CiteExportLink to record
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