Porous scaffolds from high molecular weight polyesters synthesized via enzyme-catalyzed ring-opening polymerization
2006 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 7, no 9, 2531-2538 p.Article in journal (Refereed) Published
Several aliphatic polyesters have been synthesized until now using enzyme-catalyzed ring-opening polymerization (ROP) of different lactones, although their molecular weight, hence mechanical strength, was not sufficient enough to fabricate porous scaffolds from them. To achieve this target, 1,5-dioxepan-2-one (DXO) and epsilon-caprolactone (CL) were polymerized in bulk with Lipase CA as catalyst at 60 degrees C, and porous scaffolds were prepared from the polymers obtained thereof using a salt leaching technique. The CL/DXO molar feed ratio was varied from 1.5 to 10, and the reactivity ratios of CL and DXO were determined using the Kelen-Tudos method under such conditions of polymerization. NMR results showed a slightly lower CL/DXO molar ratio in the copolymers than in the feed due to high reactivity of DXO toward Lipase CA catalysis. The crystallinity of the PCL segment of the copolymers was affected by the presence of soft and amorphous DXO domains. The copolymers having high CL content were thermally more stable. The porosity of the scaffolds was in the range 82-88%, and the SEM analysis showed interconnected pores in the scaffolds. Of the two parameters which could affect the mechanical properties, viz., the copolymer composition and the scaffold pore size, the pore size showed a significant effect on the mechanical properties of the scaffolds. The porous scaffolds developed in this way for tissue engineering are free from toxic organometallic catalyst residues, and they are highly suitable for biomedical applications.
Place, publisher, year, edition, pages
2006. Vol. 7, no 9, 2531-2538 p.
Aliphatic polyester; Enzyme-catalyzed ring-opening polymerization; Kelen-Tudos method; Salt leaching technique; Composition; Enzyme kinetics; Leaching; Molecular weight distribution; Nuclear magnetic resonance spectroscopy; Porosity; Ring opening polymerization; Scanning electron microscopy; Synthesis (chemical); Polyesters; 1, 5 dioxepan 2 one; aliphatic compound; copolymer; lactone; lipase B; organometallic compound; polycaprolactone; polyester; pyran derivative; unclassified drug; article; biomedicine; carbon nuclear magnetic resonance; catalysis; chemical composition; chemical structure; controlled study; gel permeation chromatography; leaching; mathematical computing; molecular weight; polymerization; porosity; priority journal; proton nuclear magnetic resonance; ring opening; scanning electron microscopy; synthesis; temperature measurement; tensile strength; thermogravimetry; thermostability; tissue engineering; Biocompatible Materials; Biotechnology; Catalysis; Chromatography; Lipase; Magnetic Resonance Spectroscopy; Microscopy, Electron, Scanning; Molecular Weight; Polyesters; Polymers; Salts; Tensile Strength; Thermodynamics; Thermogravimetry; Tissue Engineering
IdentifiersURN: urn:nbn:se:kth:diva-7008DOI: 10.1021/bm060309wISI: 000240403300011ScopusID: 2-s2.0-33749547412OAI: oai:DiVA.org:kth-7008DiVA: diva2:11882
QC 201008182007-04-202007-04-202010-08-18Bibliographically approved