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The role of Glu87 and Trp89 in the lid of Humicola lanuginosa lipase
KTH, School of Biotechnology (BIO), Biochemistry (closed 20130101).ORCID iD: 0000-0002-2993-9375
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1996 (English)In: Protein Engineering, ISSN 0269-2139, E-ISSN 1460-213X, Vol. 9, no 6, p. 519-524Article in journal (Refereed) Published
Abstract [en]

The importance of Glu87 and TRp89 in the lid of Humicola lanuginosa lipase for the hydrolytic activity at the water/lipid interface was investigated by site-directed mutagenesis. It was found that the effect on the hydrolytic activity upon the replacement of Trp89 with Phe, Leu, Gly or Glu was substrate dependent, The Trp89 mutants displayed an altered chain length specificity towards triglycerides, with a higher relative activity towards triacetin and trioctanoin compared with tributyrin, Trp89 was shown to be less important in the hydrolysis of vinyl esters compared with ethyl esters and triglycerides. An exclusive effect on the acylation reaction rate by the mutation of Trp89 was consistent with the data, It is suggested that Trp89 is important in the process of binding the acyl chain of the substrate into the active site for optimal acylation reaction rate, The Trp89Phe mutation resulted in an increased hydrolytic activity towards 2-alkylalkanoic acid esters. This is suggested to be due to reduction of unfavourable van der Waals contacts between Trp89 and the 2-substituent of the substrate, Thus, in contrast to natural substrates, Trp89 has a negative impact on the catalytic efficiency when substrates with bulky acyl chains are used, In contrast to the Trp89 mutations, the effect on the hydrolytic activity of the Glu87Ala mutation was almost substrate independent, 35-70% activity of wild-type lipase, A reduction of both the acylation and deacylation reaction was consistent with the data.

Place, publisher, year, edition, pages
ROYAL INST TECHNOL,DEPT BIOCHEM & BIOTECHNOL,S-10044 STOCKHOLM,SWEDEN. NOVO NORDISK AS,DK-2880 BAGSVAERD,DENMARK.: OXFORD UNIV PRESS UNITED KINGDOM , 1996. Vol. 9, no 6, p. 519-524
Keywords [en]
emulsion, hydrolase, kinetics, mutagenesis, substrate specificity
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-225146DOI: 10.1093/protein/9.6.519ISI: A1996UW13900008PubMedID: 8862552OAI: oai:DiVA.org:kth-225146DiVA, id: diva2:1194360
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QCR 20180405

Available from: 2018-04-01 Created: 2018-04-01 Last updated: 2018-04-05Bibliographically approved

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