Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
ON THE INTERFACIAL ACTIVATION OF CANDIDA-ANTARCTICA LIPASE-A AND LIPASE-B AS COMPARED WITH HUMICOLA-LANUGINOSA LIPASE
KTH, School of Biotechnology (BIO), Biochemistry (closed 20130101).ORCID iD: 0000-0002-2993-9375
1995 (English)In: BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, ISSN 0005-2760, Vol. 1258, no 3, p. 272-276Article in journal (Refereed) Published
Abstract [en]

The interfacial activation of Candida antarctica lipase A (CALA) and B (CALB) has been investigated and compared with that of Humicola lanuginosa lipase (HLL). CALB displayed no interfacial activation towards p-nitrophenyl butyrate (PNPB) when exceeding the solubility limit of the substrate. No activation was observed towards p-nitrophenyl acetate (PNPA) at the addition of sodium dodecyl sulfate (SDS) nor in the presence of a solid polystyrene surface. The catalytic action of CALB was very different from that of Humicola lanuginosa lipase, which showed a pronounced interfacial activation with the same substrates. The basis for the anomalous behaviour of CALB is proposed to be due to the absence of a lid that regulates the access to the active site. in contrast to CALB, CALA expressed interfacial activation, but the activation was not as prominent as for Humicola lanuginosa lipase (HLL). The structural basis for the activation of CALA is unknown.

Place, publisher, year, edition, pages
ROYAL INST TECHNOL,DEPT BIOCHEM & BIOTECHNOL,S-10044 STOCKHOLM,SWEDEN.: ELSEVIER SCIENCE BV , 1995. Vol. 1258, no 3, p. 272-276
Keyword [en]
EMULSION, WATER SOLUBLE, P-NITROPHENYL ESTER, ADSORPTION, SODIUM DODECYL SULFATE
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-225147DOI: 10.1016/0005-2760(95)00131-UISI: A1995RY99400008PubMedID: 7548197OAI: oai:DiVA.org:kth-225147DiVA, id: diva2:1194361
Note

QCR 20180405

Available from: 2018-04-01 Created: 2018-04-01 Last updated: 2018-04-05Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
MARTINELLE, M
By organisation
Biochemistry (closed 20130101)
Biochemistry and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 15 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf