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Human embryonic stem cell dispersion in electrospun PCL fiber scaffolds by coating with laminin-521 and E-cadherin-Fc
KTH, School of Biotechnology (BIO). (Cell Technology Group (CETEG))
KTH, School of Biotechnology (BIO). (Cell Technology Group (CETEG))
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2018 (English)In: Journal of Biomedical Materials Research. Part B - Applied biomaterials, ISSN 1552-4973, E-ISSN 1552-4981, Vol. 106, no 3, p. 1226-1236Article in journal (Refereed) Published
Abstract [en]

Advances in human pluripotent cell cultivation and differentiation protocols have led to production of stem cell-derived progenitors as a promising cell source for replacement therapy. Three-dimensional (3-D) culture is a better mimic of the natural niche for stem cells and is widely used for disease modeling. Here, we describe a nonaggregate culture system of human embryonic stem cells inside electrospun polycaprolactone (PCL) fiber scaffolds combined with defined extracellular proteins naturally occurring in the stem cell niche. PCL fiber scaffolds coated with recombinant human laminin-521 readily supported initial stem cell attachment and growth from a single-cell suspension. The combination of recombinant E-cadherin-Fc and laminin-521 further improved cell dispersion rendering a uniform cell population. Finally, we showed that the cells cultured in E-cadherin-Fc- and laminin-521-coated PCL scaffolds could differentiate into all three germ layers. Importantly, we provided a chemically defined 3-D system in which pluripotent stem cells grown and differentiated avoiding the formation of cell aggregates.

Place, publisher, year, edition, pages
Wiley , 2018. Vol. 106, no 3, p. 1226-1236
Keywords [en]
pluripotent stem cells, electrospun scaffold, PCL, three-dimensional culture, extracellular matrix coating
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-225172DOI: 10.1002/jbm.b.33928ISI: 000427068200030PubMedID: 28577328Scopus ID: 2-s2.0-85020120426OAI: oai:DiVA.org:kth-225172DiVA, id: diva2:1195006
Funder
EU, FP7, Seventh Framework Programme, 2-2013-601700
Note

QC 20180404

Available from: 2018-04-04 Created: 2018-04-04 Last updated: 2018-04-11Bibliographically approved

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Åstrand, Carolina

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Leino, MattiasÅstrand, CarolinaChotteau, Veronique
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CiteExportLink to record
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  • apa
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