Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Plasma soluble factor following two decades prolonged suppressive antiretroviral therapy in HIV-1-positive males A cross-sectional study
Karolinska Inst, Dept Lab Med, Div Clin Microbiol, S-14186 Huddinge, Sweden..
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab. Karolinska Inst, Dept Lab Med, Div Clin Microbiol, S-14186 Huddinge, Sweden..
Karolinska Univ Hosp, Karolinska Inst, Dept Med Huddinge, Unit Infect Dis, Stockholm, Sweden..
KTH, Centres, Science for Life Laboratory, SciLifeLab. KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. Karolinska Inst, Dept Lab Med, Div Clin Microbiol, S-14186 Huddinge, Sweden..
2018 (English)In: Medicine (Baltimore, Md.), ISSN 0025-7974, E-ISSN 1536-5964, Vol. 97, no 5, article id e9759Article in journal (Refereed) Published
Abstract [en]

Acute human immunodeficiency virus (HIV) infection is associated with a marked induction of several pathways that are linked to inflammation and CD4(+) T-cell depletion. Many of these processes do not fully resolve on short-term combination antiretroviral therapy (cART) (<5 years), despite complete and durable suppression of viremia. The effects of long-term (>15 years) successful antiretroviral therapy (ART) and the linkage between levels of biomarkers remain unclear. Therefore, the present study aims to assess the host plasma proteome in a well-defined clinical material from HIV-1-positive male patients on successful long-term ART (>15 years) and compared them with age-matched healthy controls and treatment-naive male patients with viremia in a cross-sectional manner. Plasma samples were obtained from 3 categories of age-matched HIV-1-positive male patients on long-term successfully (ART, n=10) with a median (Interquartile range, IQR) of 19 (17-20) years, treatment-naive patients with viremia (VP, n=14), and HIV-1-negative persons (HC, n=11). Plasma proteome was analyzed using the proximity extension assay targeting 92 factors. Statistical analyses were performed with GraphPad Prism v7, R-packages, and Qlucore Omics Explorer v3.2. Functional enrichment analysis was performed by Kyoto Encyclopedia of Genes and Genomes (KEGG), and interactions of specific molecules were identified using Path Designer integrated into Ingenuity Pathway Analysis (IPA). Group wise comparison identified 53 soluble factors, which differed between the groups (P < .05). Cluster analysis identified 13 discrete soluble factors (CD8A, CRTAM, CXCL13, EGF, CD5, CD40, CXCL9, Gal-1, IL12RB1, KLRD1, PD-1, CASP-8 and TNFRSF9) between the studied groups (adjusted P < .001). The long-term successfully ART-treated individuals clustered and networked with the HC while VPs clustered separately. All of the proinflammatory cytokines and chemokines were normalized back to levels of healthy controls in long-term successfully ART-treated individuals, but not the levels of KLRD1 and PGDFB. sKLRD1 that is involved in the regulation of natural killer cell (NK) mediated cytotoxicity, failed to be restored to the level of HIV-negative individuals despite successful long-term ART. Additional analysis of NK cells along with T-cell subsets can provide insights into the long-term effects of ART on the immune system.

Place, publisher, year, edition, pages
LIPPINCOTT WILLIAMS & WILKINS , 2018. Vol. 97, no 5, article id e9759
Keywords [en]
chemokines, cytokines, decade long ART, HIV-1
National Category
Clinical Medicine
Identifiers
URN: urn:nbn:se:kth:diva-225748DOI: 10.1097/MD.0000000000009759ISI: 000428565900024PubMedID: 29384862Scopus ID: 2-s2.0-85041676992OAI: oai:DiVA.org:kth-225748DiVA, id: diva2:1196473
Funder
Swedish Research Council, 2017-01330Stockholm County Council, ALF 20160074
Note

QC 20180410 Forskningsfinansiärer och strategiska forskningsprojekt: Jonas Soderquist's Stipendium for Experimental Virology and Immunology Research

Available from: 2018-04-10 Created: 2018-04-10 Last updated: 2018-05-23Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Zhang, WangNeogi, Ujjwal
By organisation
Proteomics and NanobiotechnologyScience for Life Laboratory, SciLifeLab
In the same journal
Medicine (Baltimore, Md.)
Clinical Medicine

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 10 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf