High Throughput Synthesis and Analysis of Acylated Cyanohydrins
2007 (English)In: Chemistry - A European Journal, ISSN 0947-6539, E-ISSN 1521-3765, Vol. 13, no 15, 4334-4341 p.Article in journal (Refereed) Published
The yields and optical purities of products obtained from chiral Lewis acid/Lewis base-catalysed additions of alpha-ketonitriles to prochiral aldehydes could be accurately determined by an enzymatic method. The amount of remaining aldehyde was determined after its reduction to an alcohol, whilst the two product enantiomers were analysed after subsequent hydrolysis first by the (S)-selective Candida antarctica lipase B and then by the unselective pig liver esterase. The method could be used for analysis of products obtained from a number of aromatic aldehydes and aliphatic ketonitriles. Microreactor technology was successfully combined with high-throughput analysis for efficient catalyst optimization.
Place, publisher, year, edition, pages
2007. Vol. 13, no 15, 4334-4341 p.
asymmetric synthesis; cyanohydrins; enantioselectivity; enzymes; high-throughput screening; microreactors
IdentifiersURN: urn:nbn:se:kth:diva-7108DOI: 10.1002/chem.200601638ISI: 000246879100022ScopusID: 2-s2.0-34250318478OAI: oai:DiVA.org:kth-7108DiVA: diva2:12021
QC 201008092007-05-152007-05-152010-08-09Bibliographically approved