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Insights on the distribution of substitutions in spruce galactoglucomannan and its derivatives using integrated chemo-enzymatic deconstruction, chromatography and mass spectrometry
Abo Akad Univ, Johan Gadolin Proc Chem Ctr, Lab Wood & Paper Chem, Porthansgatan 3-5, FI-20500 Turku, Finland.;Jiangsu Univ, Dept Environm & Safety, Biofuels Inst, Zhenjiang 212013, Peoples R China..ORCID iD: 0000-0001-5393-3187
Abo Akad Univ, Johan Gadolin Proc Chem Ctr, Lab Wood & Paper Chem, Porthansgatan 3-5, FI-20500 Turku, Finland..
Abo Akad Univ, Johan Gadolin Proc Chem Ctr, Lab Wood & Paper Chem, Porthansgatan 3-5, FI-20500 Turku, Finland..
Abo Akad Univ, Johan Gadolin Proc Chem Ctr, Lab Wood & Paper Chem, Porthansgatan 3-5, FI-20500 Turku, Finland..
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2018 (English)In: International Journal of Biological Macromolecules, ISSN 0141-8130, E-ISSN 1879-0003, Vol. 112, p. 616-625Article in journal (Refereed) Published
Abstract [en]

Accurate determination of the distribution of substitutions in the primary molecular structure of heteropolysaccharides and their derivatives is a prerequisite for their increasing application in the pharmaceutical and biomedical fields, which is unfortunately hindered due to the lack of effective analytical techniques. Acetylated galactoglucomannan (GGM) is an abundant plant polysaccharide as the main hemicellulose in softwoods, and therefore constitutes an important renewable resource from lignocellulosic biomass for the development of bioactive and functional materials. Here we present a methodology for profiling the intramolecular structure of spruce GGM and its chemical derivatives (cationic, anionic, and benzoylated) by combining chemo-enzymatic hydrolysis, liquid chromatography, and mass spectrometry. Fast identification and qualitative mass profiling of GGM and its derivatives was conducted using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF-MS) and electrospray ionization mass spectrometry (ESI-MS). Tandem mass fragmentation analysis and its hyphenation with hydrophilic interaction liquid chromatography (HILIC-ESI-MS/MS) provide further insights on the substitution placement of the GGM oligosaccharides and its derivatives. This method will be useful in understanding the structure-function relationships of native GGM and their derivatives, and therefore facilitate their potential application. 

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV , 2018. Vol. 112, p. 616-625
Keywords [en]
Plant polysaccharides, Molecular structure, Mass spectrometry, Glycomics, Chemo-enzymatic hydrolysis
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-227733DOI: 10.1016/j.ijbiomac.2018.01.219ISI: 000430522400073PubMedID: 29408392Scopus ID: 2-s2.0-85041510134OAI: oai:DiVA.org:kth-227733DiVA, id: diva2:1205761
Funder
Swedish Research Council, 621-2014-5295
Note

QC 20180515

Available from: 2018-05-15 Created: 2018-05-15 Last updated: 2018-05-15Bibliographically approved

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