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Enabling the synthesis of medium chain alkanes and 1-alkenes in yeast
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2017 (English)In: Metabolic engineering, ISSN 1096-7176, E-ISSN 1096-7184, Vol. 44, p. 81-88Article in journal (Refereed) Published
Abstract [en]

Microbial synthesis of medium chain aliphatic hydrocarbons, attractive drop-in molecules to gasoline and jet fuels, is a promising way to reduce our reliance on petroleum-based fuels. In this study, we enabled the synthesis of straight chain hydrocarbons (C7–C13) by yeast Saccharomyces cerevisiae through engineering fatty acid synthases to control the chain length of fatty acids and introducing heterologous pathways for alkane or 1-alkene synthesis. We carried out enzyme engineering/screening of the fatty aldehyde deformylating oxygenase (ADO), and compartmentalization of the alkane biosynthesis pathway into peroxisomes to improve alkane production. The two-step synthesis of alkanes was found to be inefficient due to the formation of alcohols derived from aldehyde intermediates. Alternatively, the drain of aldehyde intermediates could be circumvented by introducing a one-step decarboxylation of fatty acids to 1-alkenes, which could be synthesized at a level of 3 mg/L, 25-fold higher than that of alkanes produced via aldehydes.

Place, publisher, year, edition, pages
Academic Press Inc. , 2017. Vol. 44, p. 81-88
Keywords [en]
1-Alkenes, Alkanes, Medium-chain fatty acids, Saccharomyces cerevisiae, Aldehydes, Biochemistry, Carboxylation, Chains, Cytology, Hydrocarbons, Paraffins, Synthesis (chemical), Yeast, Aliphatic hydrocarbons, Biosynthesis pathways, Enzyme engineering, Microbial synthesis, Two-step synthesis, Yeast Saccharomyces cerevisiae, Fatty acids, 1 alkene, alcohol derivative, aldehyde, aldehyde deformylating oxygenase, aliphatic hydrocarbon, alkane, alkene, fatty acid, fatty acid synthase, medium chain fatty acid, oxygenase, unclassified drug, Article, biosynthesis, controlled study, decarboxylation, nonhuman, peroxisome, priority journal, protein expression
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Organic Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-227064DOI: 10.1016/j.ymben.2017.09.007Scopus ID: 2-s2.0-85030102515OAI: oai:DiVA.org:kth-227064DiVA, id: diva2:1206662
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QC 20180517

Available from: 2018-05-17 Created: 2018-05-17 Last updated: 2018-05-17Bibliographically approved

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Nielsen, Jens

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