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Tunable Ampere phase plate for low dose imaging of biomolecular complexes
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2018 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 8, no 1, article id 5592Article in journal (Refereed) Published
Abstract [en]

A novel device that can be used as a tunable support-free phase plate for transmission electron microscopy of weakly scattering specimens is described. The device relies on the generation of a controlled phase shift by the magnetic field of a segment of current-carrying wire that is oriented parallel or antiparallel to the electron beam. The validity of the concept is established using both experimental electron holographic measurements and a theoretical model based on Ampere's law. Computer simulations are used to illustrate the resulting contrast enhancement for studies of biological cells and macromolecules.

Place, publisher, year, edition, pages
Nature Publishing Group, 2018. Vol. 8, no 1, article id 5592
National Category
Other Engineering and Technologies
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URN: urn:nbn:se:kth:diva-227500DOI: 10.1038/s41598-018-23100-3ISI: 000429095600001PubMedID: 29618785Scopus ID: 2-s2.0-85044965282OAI: oai:DiVA.org:kth-227500DiVA, id: diva2:1207053
Funder
EU, FP7, Seventh Framework Programme, 320832
Note

QC 20180518

Available from: 2018-05-18 Created: 2018-05-18 Last updated: 2018-05-18Bibliographically approved

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Öktem, Ozan

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