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beta-(1 -> 6)-d-glucan secreted during the optimised production of exopolysaccharides by Paecilomyces variotii has immunostimulatory activity
Univ Estadual Oeste Parana, Ctr Ciencias Biol & Saude, Caixa Postal 711, BR-85819110 Cascavel, PR, Brazil..
Univ Estadual Oeste Parana, Ctr Ciencias Medicas & Farmaceut, Caixa Postal 711, BR-85819110 Cascavel, PR, Brazil..
Univ Estadual Oeste Parana, Ctr Ciencias Biol & Saude, Caixa Postal 711, BR-85819110 Cascavel, PR, Brazil..
Univ Sao Paulo, Fac Ciencias Farmaceut, BR-05508000 Sao Paulo, SP, Brazil..
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2018 (English)In: Antonie van Leeuwenhoek. International Journal of General and Molecular Microbiology, ISSN 0003-6072, E-ISSN 1572-9699, Vol. 111, no 6, p. 981-994Article in journal (Refereed) Published
Abstract [en]

Paecilomyces variotii is a filamentous fungus that occurs worldwide in soil and decaying vegetation. Optimization of the fermentation process for exopolysaccharide (EPS) production from the fungus P. variotii, structure determination and immuno-stimulating activity of EPS were performed. Response surface methodology (RSM) coupled with central composite design (CCD) was used to optimize the physical and chemical factors required to produce EPS in submerged fermentation. Preliminary investigations to choose the three factors for the present work were made using a factorial experimental design. Glucose, ammonium nitrate (NH4NO3) and pH were used as variables for which, with constant temperature of 28 A degrees C and agitation of 90 rpm, the optimal process parameters were determined as glucose values of 0.96%, NH4NO3 0.26% and pH 8.0. The three parameters presented significant effects. In this condition of culture, the main composition of the isolated EPS was a linear beta-(1 -> 6)-linked-D-glucan, as determined by Nuclear Magnetic Resonance (NMR) and methylation analysis. This polysaccharide is a very unusual as an EPS from fungi, especially a filamentous fungus such as P. variotii. Murine peritoneal macrophages cultivated with beta-glucan for 6 and 48 h showed an increase in TNF-alpha, IL-6 and nitric oxide release with increased polysaccharide concentrations. Therefore, we conclude that the beta-(1 -> 6)-linked-D-glucan produced in optimised conditions of P. variotii cultivation has an immune-stimulatory activity on murine macrophages.

Place, publisher, year, edition, pages
Springer, 2018. Vol. 111, no 6, p. 981-994
Keywords [en]
beta-Glucan, Polysaccharides secreted, Filamentous fungus, Immunoactivity, Optimisation
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Identifiers
URN: urn:nbn:se:kth:diva-229006DOI: 10.1007/s10482-017-1000-xISI: 000432108600019PubMedID: 29236232Scopus ID: 2-s2.0-85038013427OAI: oai:DiVA.org:kth-229006DiVA, id: diva2:1211605
Conference
3rd Meeting of the Planctomycetes-Verrucomicrobia-Chlamydiae Superphylum Community, MAY 10-12, 2017, Porto, Portugal
Note

QC 20180531

Available from: 2018-05-31 Created: 2018-05-31 Last updated: 2018-05-31Bibliographically approved

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