Confirmative electric DNA array-based test for food poisoning Bacillus cereus
2007 (English)In: Journal of Microbiological Methods, ISSN 0167-7012, E-ISSN 1872-8359, Vol. 70, no 1, 55-64 p.Article in journal (Refereed) Published
Detection of the full set of toxin encoding genes involved in gastrointestinal diseases caused by B. cereus was performed. Eight genes determining the B. cereus pathogenicity, which results in diarrhea or emesis, were simultaneously evaluated on a 16-position electrical chip microarray. The DNA analyte preparation procedure comprising first 5 min of ultrasonic treatment, DNA extraction, and afterwards an additional 10 min sonication, was established as the most effective way of sample processing. No DNA amplification step prior to the analysis was included. The programmed assay was carried out within 30 min, once the DNA analyte from 10(8) bacterial cells, corresponding to one agar colony, was subjected to the assay. In general, this work represents a mature analytical way for DNA review. It can be used under conditions that require almost immediate results.
Place, publisher, year, edition, pages
2007. Vol. 70, no 1, 55-64 p.
B. cereus; toxin genes; confirmative test; electrical DNA-array
IdentifiersURN: urn:nbn:se:kth:diva-7218DOI: 10.1016/j.mimet.2007.03.011ISI: 000248109500007ScopusID: 2-s2.0-34250786075OAI: oai:DiVA.org:kth-7218DiVA: diva2:12160
QC 201008242007-05-292007-05-292010-08-24Bibliographically approved