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The development of novel methods for tumor spheroids fomation and evaluation of spheroids as anti-cancer drugs treatment model
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH).
2018 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

The tumor spheroids model is thought to hold great potential on presenting heterogeneous genetic information of tumor cells and being able to provide adequate information for tumor cell behavior in terms of the cell proliferation, cell aggregation and the extracellular interaction. In this thesis project, a novel biodegradable polymer film is built-in various substrate surfaces, followed by spheroids culture characterization. Different coating modification values, different culture surfaces, multiple cell seeding concentration and two cell lines have been used to evaluate and characterize the spheroids culture system as variable factors.

In this report, the evaluation and the characterization of spheroids culture on the biodegradable polymers film coating system have been analyzed. The effect of each variable factor on the tumor spheroids formation has been discussed through the analysis of spheroids generation number on the biodegradable polymer film and the growing of spheroids size overtime correlated to the change of biodegradable polymer film assembly parameters on the culture surface.

Tumor spheroids culture characterization is also achieved in correlation to the biodegradable polymer film surface coating, suggested that a potential alternative to enhance tumor spheroids culture strategy.

Abstract [sv]

Tumör sfäroidmodellen antas ha stor potential för att presentera heterogen genetisk information av tumörceller och kunna tillhandahålla adekvat information för tumörcellers beteende i termer av cellproliferation, cellaggregation och extracellulär interaktion. I denna studie byggs en ny biologiskt nedbrytbar polymerfilm in i olika substratytor, följt av             sfärisk kulturkarakterisering. Olika beläggningsmodifieringsvärden, odlingsytor, cellodlings koncentrationer och två cellinjer har använts för att utvärdera och karakterisera sfäroidkultursystemet som variabla faktorer.

Denna studie har analyserat utvärderingen och karaktäriseringen av sfäroidkulturen på biologiskt nedbrytbara polymerers filmbeläggningssystem. Effekten av varje variabel faktor på tumör sfäroidbildningen har diskuterats genom analys av sfäroidgenerationsnummer på den biologiskt nedbrytbara polymerfilmen och tillväxten av sfäroidstorlek över tid korrelerad med förändringen av biologiskt nedbrytbara polymerfilmmonteringsparametrar på odlingsytan.

Tumör sfäroidkulturkarakterisering uppnås också i korrelation med ytbeläggningen av biologiskt nedbrytbar polymerfilm, vilket visar ett potentiellt alternativ för att förbättra odlingsstrategin för tumör sfärer.

Place, publisher, year, edition, pages
2018.
Keywords [en]
Biodegradable polymer film, tumor spheroids, HEK 293 T, HCT 116, coating modification parameter, formation rate, size deviation, anti-cancer drug screening.
National Category
Other Engineering and Technologies
Identifiers
URN: urn:nbn:se:kth:diva-230773OAI: oai:DiVA.org:kth-230773DiVA, id: diva2:1219379
Available from: 2018-06-21 Created: 2018-06-15 Last updated: 2018-06-21Bibliographically approved

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