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Development of a lab-scale drinking water treatment process using magnetic nanoparticles functionalized with protein from Mo ringa oleifera
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH).
2018 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Clean and safe drinking water is essential due to health-aspects and quality of life for all humans on earth. Implementation and development of easy-to-use, high-quality and cheap methods for water treatment is something that is of very high importance. Alternative methods to conventional water treatment used in e.g. Sweden could be to use plant material for the coagulation step. In this study crude plant protein from Moringa oleifera (MOCP) was utilized for its coagulant properties to flocculate particles in turbid waters. To make the process more e cient the crude plant protein was functionalized with magnetic nanoparticles (MNP) which enabled fast and easy separation from the treated water with the help of an external magnet, the complex formed was called MNP- MOCP. Development of a prototype water treatment process of more than one litre was made using MNP-MOCP where aluminum sulphate was used as control since it is used in conventional water treatment plants in Sweden. The process was optimized for magnetic separation, mixing and settling time. Other parameters tested in the process were to try di↵erent turbidity waters, antimicrobial properties of MNP-MOCP in larger scale and lowering of sludge water content when using the MNP-MOCP method. Furthermore, a general feasibility study was conducted in parallel with the process development to calculate the costs of the material used, the energy demand and the cost to treat one cubic meter of water with MNP-MOCP. The coagulation activity was measured for all processes with a spectrophotometer at 500 nm and a turbidimeter for lower turbidity values, using aluminum sulphate as the control. Dry weight was measured for sludge and treated water with MNP-MOCP to test if the recovery of particles with an external magnet was su cient. The results showed that it is possible to treat water up to 1 litre with MNP-MOCP and obtain a coagulation activity of at least 90 % after a total of 20 minutes from start to finish in both high and low turbidity waters. The process had high coagulation activity in lower turbidity (5.3-50 NTU) as well, which would imply that MNP-MOCP could be be used as a coagulant in Sweden where low turbidity waters are common. Furthermore, the results shows that growth inhibition of gram negative and gram positive bacteria occurred, but further studies needs to be conducted on that. Feasibility study shows that it would be feasible to use MNP-MOCP due to its short process time and low energy demand. The possibility to regenerate and further optimize the process shows great prospects for the method in the future. 

Place, publisher, year, edition, pages
2018.
National Category
Other Engineering and Technologies
Identifiers
URN: urn:nbn:se:kth:diva-233475OAI: oai:DiVA.org:kth-233475DiVA, id: diva2:1240231
Available from: 2018-08-20 Created: 2018-08-20 Last updated: 2018-08-20Bibliographically approved

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