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Using fluorescent probes and FRAP to investigate macromolecule diffusion in steam-exploded wood
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Centres, Wallenberg Wood Science Center. Chalmers Univ Technol, Dept Chem & Chem Engn, S-41296 Gothenburg, Sweden.;Chalmers Univ Technol,;Chalmers Univ Technol, SuMo Biomat, Gothenburg, Sweden..
RISE Agrifood & Biosci, Prod Design & Percept, Box 5401, S-40229 Gothenburg, Sweden.;Chalmers Univ Technol, SuMo Biomat, Gothenburg, Sweden..
Chalmers Univ Technol, Dept Chem & Chem Engn, S-41296 Gothenburg, Sweden.;RISE Agrifood & Biosci, Prod Design & Percept, Box 5401, S-40229 Gothenburg, Sweden.;Chalmers Univ Technol, SuMo Biomat, Gothenburg, Sweden..
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Centres, Wallenberg Wood Science Center. Chalmers Univ Technol, Dept Chem & Chem Engn, S-41296 Gothenburg, Sweden.;Chalmers Univ Technol, .
2018 (English)In: Wood Science and Technology, ISSN 0043-7719, E-ISSN 1432-5225, Vol. 52, no 5, p. 1395-1410Article in journal (Refereed) Published
Abstract [en]

Diffusion of fluorescently labeled dextran of varying molecular weight in wood pretreated by steam explosion was studied with a confocal microscope. The steam explosion experiments were conducted at relatively mild conditions relevant for materials biorefinery at a pressure of 14 bars for 10 min. The method of fluorescence recovery after photobleaching (FRAP) was used to perform diffusion measurements locally in the wood microstructure. It was found that the FRAP methodology can be used to observe differences in the diffusion coefficient based on localization in the microstructure, i.e., earlywood, latewood, and cell wall. Microscopic changes due to steam explosion were seen to increase diffusion of the smaller 3-kDa dextran diffusion probe in the earlywood, while the latewood structure was not affected in any significant way. Macroscopic changes to the structure in the form of ruptures due to the steam explosion pretreatment were observed to increase the rate of diffusion for the larger 40-kDa dextran probe.

Place, publisher, year, edition, pages
SPRINGER , 2018. Vol. 52, no 5, p. 1395-1410
National Category
Materials Engineering
Identifiers
URN: urn:nbn:se:kth:diva-233590DOI: 10.1007/s00226-018-1039-5ISI: 000441288200014Scopus ID: 2-s2.0-85050675818OAI: oai:DiVA.org:kth-233590DiVA, id: diva2:1242367
Note

QC 20180828

Available from: 2018-08-28 Created: 2018-08-28 Last updated: 2018-08-28Bibliographically approved

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