Lack of association between a cationic protein and a cationic fluorosurfactant
2007 (English)In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 23, no 2, 771-775 p.Article in journal (Refereed) Published
Surface tension, F-19 and H-1 NMR spectroscopy, and cryotransmission electron microscopy are used to characterize the state of association in aqueous solutions of a fluorosurfactant CF3(CF2)(n)SO2NH(CH2)(3-4)N(CH3)(3)(+) I- (n = 8, 6) with and without lysozyme added. In the absence of lysozyme, we find monomers, small aggregates, and large vesicles to coexist, with the individual fluorosurfactant molecules exchanging slowly (> 1 ms) among those states. When both lysozyme and fluorosurfactant are present in the solution, they have no measurable influence on the physical state of the other. In contrast, a hydrogenated cationic surfactant with the same headgroup, hexadecyltrimethylammonium bromide, is shown to associate to lysozyme.
Place, publisher, year, edition, pages
2007. Vol. 23, no 2, 771-775 p.
Fluorescence; Monomers; Nuclear magnetic resonance; Proteins; Solutions; Surface tension; Transmission electron microscopy; Cationic fluorosurfactants; Cationic proteins; Cryotransmission electron microscopy; Individual fluorosurfactant molecules; Surface active agents; cation; cetrimide; lysozyme; surfactant; article; chemical structure; chemistry; cryoelectron microscopy; hydrogen bond; methodology; nuclear magnetic resonance spectroscopy; physical chemistry; protein folding; surface property; surface tension; transmission electron microscopy; Cations; Cetrimonium Compounds; Chemistry, Physical; Cryoelectron Microscopy; Hydrogen Bonding; Magnetic Resonance Spectroscopy; Microscopy, Electron, Transmission; Molecular Structure; Muramidase; Protein Folding; Surface Properties; Surface Tension; Surface-Active Agents
IdentifiersURN: urn:nbn:se:kth:diva-7413DOI: 10.1021/la062469zISI: 000243338500061PubMedID: 17209632ScopusID: 2-s2.0-33846852625OAI: oai:DiVA.org:kth-7413DiVA: diva2:12434
QC 201507212009-08-302009-08-302015-07-21Bibliographically approved