Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
High yield passive plasma filtration from human finger prick blood
KTH, School of Electrical Engineering and Computer Science (EECS), Micro and Nanosystems.
KTH, School of Electrical Engineering and Computer Science (EECS), Micro and Nanosystems.
Show others and affiliations
(English)Manuscript (preprint) (Other academic)
National Category
Medical Engineering
Identifiers
URN: urn:nbn:se:kth:diva-234678OAI: oai:DiVA.org:kth-234678DiVA, id: diva2:1246545
Note

QC 20180911

Available from: 2018-09-07 Created: 2018-09-07 Last updated: 2019-05-02Bibliographically approved
In thesis
1. Capillary driven devices for patient-centric diagnostics
Open this publication in new window or tab >>Capillary driven devices for patient-centric diagnostics
2018 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Lateral flow assays is an example of a successful microfluidic platform relying on passive fluid transport, making them suitable for patient-centric and point-of-care applications. Flow control and valving in capillary driven devices typically rely on design-imprinted functions and operations which can be a limiting factor. This thesis explores dissolvable polymer valves in capillary driven microfluidic systems, a novel type of valves with a timing function. The dissolvable valve technology was used to develop autonomous operations in lamination-based polymer microfluidic systems such as sequential reagent delivery, reagent release and volume-metering, and further utilizes this technology in the Dried Blood Spot (DBS) and Dried Plasma Spot applications described below. Lamination technology is suitable for the integration of the water-dissolvable polymer layers and allows upscaling at a relatively low cost. Advances in the development of LC-MS/MS systems enable the quantification of analytes in microliter-sized blood samples such as DBS. This makes DBS sampling a minimally invasive alternative to venous blood sampling with logistical and ethical advantages for users and health care providers. Unknown sample volume, spot inhomogeneity and hematocrit-related issues have been an obstacle for a wider acceptance of DBS sampling technology. To address these issues, a novel blood-sampling device, the microfluidic DBS card, has been developed within this thesis. The device function is based on capillary driven volume-metering and allows accurate and user independent collection of microliter-sized DBS, directly from a finger-prick. The microfluidic DBS card could help to eliminate some of the issues related to DBS sampling and contribute to a wider acceptance of the technology. Usability and reliability have been considered during the development to enable testing of the microfludic DBS card in a pre-clinical setting. For many analytes and biomarkers, conventional blood sample analysis is performed on plasma or serum samples. This thesis further discusses the use of capillary driven plasma separation based on commercially available asymmetric filtration membranes and capillary driven flow in microchannels. A novel concept for hematocrit and input-volume-independent collection of a 11.6~µl plasma sample from a single drop of blood is demonstrated. The plasma sample is automatically transferred to a sample collection pad forming a Dried Plasma Spot. This could be the next generation of dried sample matrix, enabling an accurate quantification of analytes in Dried Plasma Spots.

Place, publisher, year, edition, pages
Stockholm: Kungliga Tekniska högskolan, 2018. p. 81
Series
TRITA-EECS-AVL ; 2018:58
Keywords
Capillary driven, Microfluidic, Dissolvable valves, PVA, Volume metering, Dried Blood Spots, DBS, Dried Plasma Spots, DPS
National Category
Medical Engineering
Research subject
Medical Technology
Identifiers
urn:nbn:se:kth:diva-234679 (URN)978-91-7729-919-6 (ISBN)
Public defence
2018-09-28, F3, Lindstedtsvägen 26, Stockholm, 10:00 (English)
Opponent
Supervisors
Note

QC 20180910

Available from: 2018-09-10 Created: 2018-09-07 Last updated: 2018-09-10Bibliographically approved

Open Access in DiVA

No full text in DiVA

Search in DiVA

By author/editor
Hauser, JanoschLenk, GabrielStemme, GöranRoxhed, Niclas
By organisation
Micro and Nanosystems
Medical Engineering

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 229 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf